Welcome to Chinese Journal of Ecology! Today is Share:

cje ›› 2005, Vol. ›› Issue (12): 1444-1448.

Previous Articles     Next Articles

Accumulation and distribution of copper granules in shrimp Macrobrachium nipponense

LU Xingjia, XI Yilong, LIU Guiyun   

  1. College of Life Science, Anhui Normal University, Wuhu 241000, China
  • Received:2004-03-08 Revised:2005-11-10 Online:2005-12-10

Abstract: By the methods of histochemistry,transmission electron microscope(TEM) and atomic absorption spectrophotometer(AAS) analysis,this paper studied the accumulation and distribution of copper granules in the major organs of shrimp Macrobrachium nipponense exposed to 0.08 mg稬-1 Cu2+ for 10 days.The results showed that there were large amounts of high electronic dense copper granules in the hepatopancreas of M.nipponense,which primarily deposited in tubules and R cells,and then excreted to haemocyte space.Under ultrastructure,the copper granules were mainly deposited in the lysosome of hepatopancreas cells,and a less amount of them was absorbed in smooth-surfaced endoplasmic reticulum.The copper granules were excreted to the lumen by apocrine secretion.There were copper granules in midgut cells,and vacuolus occurred in the cytoplasm of cells.The cristae in mitochondria were disintegrated partially,and copper granules were absorbed into mitochondria.A little amount of copper granules was deposited in the lysosome of antennal gland cells,and endoplasmic reticulum was curved in the cells.The highest concentration of copper(546.39 mg穔g-1) was found in hepatopancreas by AAS,suggesting that hepatopancreas might play an important role in storing copper.

Key words: land uses, Hood infiltrometer, hydraulic properties, macroporosity.

CLC Number: