Abstract: Two ultrasonic wave extracts (n-butarol and ethyl acetate as solvents) of Phytolacca americana acetone extract and residues after extracting were investigated for acaricidal activities against Tetranychus cinnabarinus. Results showed that the n-butarol extract exhibited the highest acaricidal activity among all treatments. The corrected mortalities of n-butarol extract were 74% and 97% after 24 h and 48 h of treatment at 2.5 mg·mL^-1, and its LC₅₀ and LC₉₀ were 1.787 and 7.272 mg·mL^-1 for 48 h, respectively. Meanwhile, the repellent rates were 62%, 82% and 84% against the mite for 24 h and oviposition inhibition rates (48 h) were 39%, 81% and 93%, at concentrations of 4, 2 and 1 mg·mL^-1, respectively. The nbutarol extract was further separated by the silica gel column and monitored by thinlayer chromatography (TLC) with UV detection, and 14 fractions were obtained. The 11th fraction showed highest acaricidal activity with a corrected mortality of 98% at the concentration of 2 mg·mL^-1 and its LC₅₀ and LC₉₀ were 0.932 and 3.110 mg·mL^-1, respectively after 48 h of treatment.

Key words: metaproteomics, metabolic pathways, biodegradation mechanism, microbial ecosystems, biological treatment of wastewater.