Abstract: A genetic diversity analysis was made on the morphological characteristics (pupa weight, pupa length, female weight, female wing length, female body length, male weight, male wing length, and male body length) of Dendrolimus punctatus punctatus Walker, D. superans Butler, D. houi Lajonquiere, and D. kikuchii Matsumura. Significant differences were observed in the test morphological characteristics among the four Dendrolimus species, with the largest coefficient of variation (CV=51.99%) of pupa weight. D. superans population had the largest morphological diversity, while D. houi population had the smallest one. The principal component analysis showed that pupa length, female weight, and female wing length contributed most to the morphological diversity. Based on the UPGMA clustering, the first branch included D. punctatus punctatus and D. superans, and the second one included D. houi and D. kikuchii. There exited close correlations between the morphological diversity of the Dendrolimus species and the ecoenvironmental factors.