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cje ›› 2012, Vol. 31 ›› Issue (5): 1129-1135.

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Effects of reduced UV-B radiation on the variation of fluecured tobacco proteome.

CHEN Zong-yu1**, BI Ting2, WU Xiao-xiao1   

  1. (1College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan 650201, China; 2 Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200032, China)
  • Online:2012-05-10 Published:2012-05-10

Abstract: To understand the effects of different UV-B radiation intensity on the physiological metabolism and its regulatory pathways of flue-cured tobacco, twodimensional gel electrophoresis and mass spectrum technique was adopted to study the physiological traits and protein expression profiles of Nicotina tobaccumL.cv. “K326”, a flue-tobacco cultivar commonly planted in Yunnan Province, under the covering of different transparent films to reduce UV-B radiation. Two treatments were installed, i.e., covering with polyethylene (treatment I, 75.8% UV-B radiation intensity) and Mylar (treatment II, 37.5% UV-B radiation intensity). In the two treatments, ten protein spots expressed significantly different. As compared in treatment I, five protein spots were upregulated while the other five were down-regulated in treatment II. Among the ten protein spots, three were redox-related, three were photosynthesisrelated, one was a kinase involved in energy metabolism, one was RNA-binding, and the other two were to be further identified. A preliminary research was also made on the effects of different UV-B radiation intensity on the physiological metabolism of “K326” at proteome level. During the physiological mature period, transition period, and process mature period of “K326”, the net photosynthetic rate (Pn) was significantly higher in treatment II than in treatment I, which was in accordance with the result mentioned above (three up-regulated proteins related with photosynthesis in treatment II). Comparatively, the “K326” in treatment I grew faster, and most of the morphological parameters such as stem girth and specific leaf mass were higher.

Key words: orchard, vegetation coverage, biological-control factor, soil erosion.