cje ›› 2012, Vol. 31 ›› Issue (06): 1468-1472.
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LIU Pu-fang, WANG Hong-yan**
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Abstract: The rhizobial DNA was extracted from the root nodules of soybean plants at a 6-year experiment site on black soil, and PCR-DGGE and clone sequencing were adopted to analyze the effects of different fertilization treatments on the diversity of soybean rhizobia. In the treatment no fertilization, the band number of nifH gene and the diversity indices were the highest, and there existed significant differences in the diversity indices among different treatments, with the order of no fertilization > high application rate of organic manure > low application rate of organic manure, 1/2 organic manure plus 1/2 chemical fertilizer > high application rate of chemical fertilizer > low application rate of chemical fertilizer. Cluster analysis demonstrated that the fingerprints in treatments of chemical fertilization only had a similarity of 66% with those in other treatments, suggesting that chemical fertilization could significantly alter the community structure of soybean rhizobia. The PCR-DGGE and clone sequencing showed that most of the rhizobia were belonged to Bradyrhizobium. The application of organic manure and chemical fertilizer, especially the latter, could decrease the diversity of soybean rhizobia.
LIU Pu-fang, WANG Hong-yan**. Effects of organic and chemical fertilizer applications on the diversity of soybean rhizobia.[J]. cje, 2012, 31(06): 1468-1472.
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URL: http://www.cje.net.cn/EN/abstract/abstract20149.shtml
http://www.cje.net.cn/EN/Y2012/V31/I06/1468