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cje ›› 2012, Vol. 31 ›› Issue (07): 1785-1790.

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Effects of di-2-ethylhexyl phthalate (DEHP) on the expression of StAR, CYP17, and CYP19 in Rana chensinensis testis.

LI Miao, ZHANG Yu-hui**   

  1. (College of Life Science, Shaanxi Normal University, Xi’an 710062, China)
  • Online:2012-07-10 Published:2012-07-10

Abstract: In order to investigate the effects of di-2-ethylhexyl phthalate (DEHP) on the steroid hormone synthesis in amphibian testis, the male adults of Rana chensinensis were exposed to 10-7, 10-6, 10-5 and 10-4 mol·L-1 of DEHP for 20, 30 and 40 days, respectively, and the total RNA in the testis was extracted, the cDNA synthesis was performed by reverse transcription, and the relative mRNA expression values of StAR,CYP17 and CYP19 were determined by fluorescence real-time quantitative PCR. As compared with the control, the expression of StAR and CYP19 was up-regulated, and that of CYP17 was down-regulated. The effects of different concentration DEHP on the mRNA expression of StAR, CYP17 and CYP19 were less regular, whereas the cumulative effect of exposure time was more obvious. It was suggested that DEHP could affect the expression of related key enzymes in Rana chensinensis  testis via interfering with the expression of StAR, CYP17 and CYP19 in the testis, and then, interfere with the synthesis of steroid hormones and result in estrogen effect.

Key words: hyporheic zone, macroinvertebrates, functional feeding group, vertical distribution.