Abstract: Four treatments of water salinity (fresh water, CK; salinity 18, group A; salinity rapidly varied from 18 to 30, group B; and salinity rapidly varied from 18 to 0, group C) were installed to monitor eight behavior indicators of female parent Chinese crab (Eriocheir sinensis) by the method of video recording analysis and to measure the haemolymph osmolality, haemolymph ion concentration, and hemocyanin concentration of the female parent E. sinensis under different water salinities. The closure reaction behavior of the E. sinensis only occurred in groups A and B, and the duration of this reaction was significantly longer in group B than in group A (P<0.05). The abdomen extension behavior was only found in group C. The duration of antennule retraction in groups A and B was significantly higher than that in CK (P<0.05). The reaction frequency of other five behaviors was significantly higher in group C than in other 3 groups. The haemolymph osmolality and haemolymph ion concentration of the E. sinensis were higher in groups A, B, and C than that in the CK, and the haemolymph osmolality and haemolymph Na⁺ and Cl^- concentrations increased with increasing water salinity. On the contrary, the hemocyanin concentration increased with decreasing water salinity, being significantly higher in group C than in group B (P<0.05). It was considered that the female parent E. sinensis was in hyper osmoregulation when the water salinity was varied from 0 to 30. Abdomen extension could bring the hindgut into direct contact with the water, functioning as an additional means of ion uptake in low water salinity. Closure reaction could help the reduction of water absorption and salt loss in high salinity environment.

Key words: water footprint (WF), life cycle assessment (LCA), impacts on environment, agricultural product.