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cje ›› 2012, Vol. 31 ›› Issue (08): 2158-2162.

• Articles • Previous Articles    

Determination of Reaumuria soongorica leaf F3H and DFR activities under drought stress by using RP-HPLC-MS.

LIU Mei-ling1,2, LIU Yu-bing1**, CAO Bo1,2   

  1. (1Laboratory of Plant Stress Ecophysiology and Biotechnology, Shapotou Desert Research & Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
  • Online:2012-08-10 Published:2012-08-10

Abstract: Flavanone 3-hydroxylase (F3H) and dihydroflavonol 4-reductase (DFR) are the key enzymes in flavonoids biosynthesis pathway, and to study their activities is a crucial step to explore the regulation of this pathway. In this paper, a RP-HPLC-MS determination method of F3H and DFR activities was established to investigate the effects of drought stress on the activities of F3H and DFR in Reaumuria soongorica leaves. The calibration range of F3H substrate naringenin and of DFR substrate dihydroquertin was 0.04-1.20 μg (r=0.998) and 0.08-2.40 μg (r=0.998), respectively, suggesting that this method was convenient and accurate to determine the activities of F3H and DFR. By using this method, the F3H and DFR activities in R. soongorica leaves were measured, and the results showed that under drought stress, the F3H activity increased first and then dropped to the same level as the control, while the DFR activity increased all along with increasing drought time, indicating that drought stress had induction effect on the F3H and DFR activities in R. soongorica leaves.

Key words: potato, soybean, intercropping, photosynthetic characteristics, yield, compensation effect.