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Isolation, identification, and degradation characteristics of bacterial strains in a high concentration organic pollutants-contaminated river water.

ZHU Jian1,2, WANG Ping1,2**, LI Han-dong3, LEI Ming-jing1   

  1. (1Institute of Environmental Science and Engineering Research, Central South University of Forestry & Technology, Changsha 410004, China; 2National Engineering Laboratory for Applied Technology of Forestry & Ecology in South China, Changsha 410004, China; 3Chinese Research Academy of Environmental Sciences, Beijing 100012, China)
  • Online:2013-05-10 Published:2013-05-10

Abstract: In order to restore the self-purification capability of the northern seasonal river seriously contaminated by organic pollutants, a typical black-odor river in Hebei Province of North China was taken as the study object, its sediments and water samples were collected, and the culture media specifically for lactobacillus, yeast, actinomycetes, and nitrobacteria were adopted to enrich, isolate, and identify the microbes in the samples. A total of 15 organic pollutants-degrading bacteria strains were isolated, and 6 dominant bacterial isolates of them were identified by fatty acids map analysis, which were Flavobacterium, Micrococcus, Lactobacillus, Micromonospora, Candida, and Nitrococcus. To improve the adaptation and degradation capabilities of the isolates, a directional enrichment solution was adopted to compound the isolates, and the compound bacteria were obtained. The results of the optimization experiment showed that the optimal proportions of the compound bacteria were 0.5% of yeast, 1.0% of lactobacillus, 2.0% of actinomycetes, and 2.0% of nitrobacteria, and the most appropriate growth conditions of the compound bacteria were pH 7.0, temperature 35 ℃, carbon source glucose, and small amounts of Fe2+, Mg2+, and Ca2+. The degradation experiment indicated that when the pH value was 8.0, temperature was 35 ℃, inoculation amount was 0.5%, and rotation rate was 80 r·min-1, the compound bacteria performed best in degrading organic pollutants, with the removal rates of CODcr, NH3-N, and TP reached 78%, 59%, and 77%, respectively.

Key words: dry farming regions, dual-mulching of ridges and furrows.