Abstract: As a karst area of southern China, the Shibing in Guizhou Province is a second-batch candidate site nominated for the list of World Heritage Sites. In this paper, a threeyear survey was conducted on the fish species composition in Shibing. A total of 46 species of freshwater fishes were collected, belonging to 39 genera, 15 families, and 7 orders, among which, Cypriniformes had 28 species, accounting for 61% of the total. In species composition, native fishes dominated, with 40 species and occupying 87% of the total, whereas alien species had 6 species, occupying 13% of the total, and including 1 known invasive species (Gambusia affinis) and 5 artificial feeding species (Protosalanx hyalocroaniah, Ctenopryngodon idellus, Hypophthalmichthys molitrix, Aristichthys nobilis, and Megalobrama amblycephala).

Key words: black soil, farmland ecosystem, micro and mediumsized soil fauna, community composition, EM compost, biodiversity.