Abstract: To screen endophytic bacteria that promote the accumulation of bioactive ingredients in medicinal plants, endophytic bacteria residing Atractylodes macrocephala were cultured by pure cultured method and identified by 16S rDNA sequencing. The biochemical characteristics and effects on host plant growth were analyzed. Results showed that a total of 49 endophytic bacteria were isolated from A. macrocephala, including 11 genera. Bacillus and Pseudomonas were the dominant genera. Among the isolated strains, there were 10, 25, 19 strains with ability of nitrogen fixation, phosphorus solubilization and potassium dissolving, respectively. 35 strains were able to synthesize indole acetic acid. Bacillus endophyticus AMR83, a stain with all the above biological characteristics, can promote plant root growth, increase the volatile oil content inA. macrocephala roots by 100.55%, and adjust the proportion of active ingredients. The results suggested that AMR83 could be used as an endophyte to improve the yield of volatile oil and the quality of crude drug.

Key words: quantitative evaluation techniques, thermal infrared remote sensing, urban thermal environment, ground meteorological observation, numerical simulation.