Abstract: Sucrose is an important type of carbohydrates, the metabolism of which is closely related to plant growth, development and stress adaptation. Sucrose synthases (SUS), sucrose phosphate synthases (SPS) and sucrose invertases (INV) are three kinds of key enzymes involved in sucrose metabolism. Based on the transcriptome data, we identified 2 SUS, 2SPS and 7 INV genes (GenBank No: MK38694353) from Jerusalem artichoke, a bioenergy plant species. Bioinformatics analysis showed that SUS, SPS, and INV belonged to hydrophilic proteins, whose amino acid sequences had high similarity with other species. The three kinds of genes exhibited different expression patterns in the leaves of Jerusalem artichoke seedling treated at 25 ℃ and 30 ℃ for 10, 15, and 20 days, respectively. However, the product contents associated with sucrose metabolism, such as fructose, sucrose, and 1-kestose, had no significant changes, except for the total soluble sugar, the content of which was significantly reduced. This result indicated that these key enzyme genes responded to high temperature, and that sucrose metabolism was in a balanced state, reflecting that Jerusalem artichoke had a good tolerance ability to high temperature.

Key words: ecologicalization, industrial structure, spatio-temporal pattern, Jilin Province.