Welcome to Chinese Journal of Ecology! Today is Share:

cje

Previous Articles     Next Articles

Identification and diversity analysis of phytoplasma associated with Praxelis clematidea witch’s broom. 

LI Guang-ming1,2, YUAN Kun1, YANG Li-fu1, CHEN Qiu-bo1, WANG Zhen-hui1**   

  1. (1Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences/Danzhou Investigation & Experiment Station of Tropical Crops, Ministry of Agriculture, Danzhou 571737, Hainan, China; 2School of Environment and Plant Protection, Hainan University, Haikou 570228, China)
  • Online:2012-12-10 Published:2012-12-10

Abstract: To study the diversity and classification of phytoplasma associated with Praxelis clematidea witch’s broom is of significance for the biocontrol of the pathogen. In this paper, the P. clematidea plants with the presence of the phytoplasma were collected from eight counties in Hainan Province of South China. The samples were tested by PCR amplification using phytoplasmaspecific 16S rDNA primers. Furthermore, the diversity of the phytoplasma associated with P. clematidea witch’s broom was investigated with restricted fragment length polymorphisms (RFLP) by using the restriction enzymes Afa I, Alu I, EcoR I, Hae Ⅲ, HpaⅡ, Hha I, Hinf I, Kpn I, Sau3A I, Taq I, and Xsp I. The PCR products were cloned and sequenced, and the sequences were compared with the members of 16SrII group of phytoplasma. The results showed that the causal agent of P. clematidea witch’s broom was the phytoplasma. The patterns of RFLP analysis of 16S rDNA sequence primed from the phytoplasma collected from the eight counties were basically the same, and the similarity between the 16S rDNA and that of other known phytoplasmas ranged from 0.26 to 0.97. There was a high similarity (>99.4%) within the sequences from the eight counties, and the highest similarity (99.1%) with the phytoplasma from peanut witch’s broom. It was suggested that the phytoplasma of P. clematidea witch’s broom could be categorized as a branch of 16S rRNAⅡ-A group.