Abstract: Based on the bottom trawl survey data in 1986-2010, and by using the techniques of GIS spatial analysis and geostatistics, this paper studied the spatiotemporal distribution and its seasonal dissimilarity of anchovy (Engraulis japonicus) stock density in Yellow Sea under the double stress of fishing pressure and climate change. There existed obvious variations in the spatial distribution of the anchovy in different seasons. In winter, the stock density of the anchovy decreased gradually from the north-eastern waters to the shore and southern waters; in spring, the stock density decreased gradually from the shore to the eastern waters; in summer, the stock density had a trend of decreased from the northern waters to the southern waters, but the high value points scattered; in autumn, similar to that in winter, the stock density decreased from the eastern waters to the shore, but the variation gradient was smaller than that in winter. The stock density center of the anchovy had an obvious seasonal variation, but the variation pattern differed with years. From 1986 to 2010, the stock density center of the anchovy moved northward in spring, summer and autumn, and had an obvious yearly transformation but no overall migration trend in winter. In different seasons, the aggregation areas of the anchovy’s high stock density differed spatially.