Abstract: Based on daily observed data of 20 meteorological stations from 1960 to 2011 and historical freeze injury records in Beijing region, freeze injury index (FI) of winter wheat was established by principal component analysis. Methods of empirical orthogonal function (EOF), Morlet wavelet transform analysis and MannKendall test were used to analyze the spatialtemporal distribution characteristics of freeze injury to winter wheat during overwintering stage, and the comprehensive climatic risk index was synthesized to study the climatic risk zonation of freeze injury in the region. Historical freeze injury of winter wheat could be actually characterized by the FI in Beijing region; the greater the FI was, the more serious freeze injury would occur. In the past 52 years, the trend of annual FI was declined significantly (P<0.01), and the variation of annual FI was obviously increased but the main eigenperiod of freeze injury was tended to be shortened. The climatic risk probability of freeze injury ranged from 32.1% to 82.8% in Beijing region, and the probabilities of slight, moderate and severe freeze injury were 21.5%, 17.4% and 17.3%, respectively. The lowrisk area of freeze injury was mainly located in Changping district, while the highrisk areas of freeze injury were mainly located in southeast of Huairou, southwest of Miyun, northeast of Shunyi and Pinggu districts, and the rest of majority areas were moderaterisk zones of freeze injury in wheat production in Beijing region.