Abstract: The sensitivity of Pardosa pseudoannulata to the sound and silence sources of Drosophila wings was compared in the laboratory. At close distances, the selection index of the P. pseudoannulata for the sound source was significantly higher than that for the silence source. However, when the distance was increased to 15 cm, no significant difference was found in the selection index. The residence time also showed a similar change. Within 12 cm, the residence time of the P. pseudoannulata on the sound source side was longer than 50% of the total observation time, and significantly higher than that on the silence side. As the distance increased, the sensitivity of the P. pseudoannulata to the sound decreased, and the residence time became insignificantly different. Therefore, the closer the distance to the sound source, the higher the sensitivity of P. pseudoannulata was to the sound. In addition, the distance between sound sources showed a clear correlation with the successful positioning of the prey. P. pseudoannulata can locate their prey by relying on hearing from a certain distance. Moreover, the gender of P. pseudoannulata is a factor in the sensitivity of the auditory sensor. Female P. pseudoannulata is more sensitive to the sound of Drosophila wings and exhibits a higher selection index.