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Exogenous nitric oxide and hydrogen peroxide regulate the acclimation of chicory (Cichorium intybus) to salt stress.

GU Wen-ying1, MO Ping-hua2, YANG Jiang-shan4, ZHAO Guo-qi1, GAO Hong-wen3**   

  1. (1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China; 2 College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China; 3Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 4Barenbrug (Tianjin) INT’ Co. Ltd., Beijing 100025, China)
  • Online:2014-01-10 Published:2014-01-10

Abstract: Chicory (Cichorium intybus) seedlings were employed to study the impacts of exogenous nitric oxide (NO) donor (sodium nitroprusside (SNP), 0.1 mmol·L-1) or hydrogen peroxide (H2O2, 0.5 mmol·L-1) on their growth, antioxidant enzyme activities and stress protein expression under salt stress (210 mmol·L-1 of NaCl). The results showed that root length and fresh weight of chicory seedlings were obviously decreased under salt stress compared with the control, whereas the content of malondialdehyde (MDA) sig
nificantly increased (P<0.05). In addition, isoenzyme analysis showed that the activities of superoxide dismutase (SOD) and catalase (CAT) were weakened while peroxidase (POD) activity obviously increased under salt stress. The data from qPCR analysis suggested that the relative quantity of HSP 90 mRNA and CiDHN1 mRNA increased under salt stress, but the data from ELISA indicated that only the content of CiDHN1 gradually increased in a timedependent manner (2-48 h). Compared with the single salt stress, the inhibition on root length and fresh weight of plants induced by salt stress was apparently alleviated by SNP pretreatment, with a dramatic decline of MDA content, an obvious rise in activities of SOD, POD and CAT and an increase in numbers of SOD and POD isoenzymes. Moreover, qPCR analysis indicated that SNP pretreatment further increased the relative quantity of HSP 90 mRNA and CiDHN1 mRNA, and the contents of HSP 90 and CiDHN1 in chicory seedlings. The effects of H2O2 pretreatment were similar to those of SNP pretreatment. These data suggested that both of SNP and H2O2 were involved in alleviating adverse effects of salt stress on chicory seedlings by increasing antioxidant enzyme activities and stress protein expression.

Key words: Bothriochloa ischaemum, elevated CO2 concentration, PLFA., drought, rhizosphere effect