Abstract: To clarify the impact of temperature change on gene expression in roots, we made a plant expression vector pBIPhsp::ROS, which harbors a MYB gene Rosea1 controlling anthocyanin biosynthesis in Antirrhinum majus and the promoter of small heatshock protein 18.2 of Arabidopsis. We obtained hairy roots by genetic transformation. Moreover, we investigated the impact of temperature change on gene expression in roots. The results showed that anthocyanin accumulations were observed in the transgenic hairy roots under treatment of 37, 40 and 42 ℃ for 4 h, and the deepest coloration was detected in the treatment of 42 ℃ for 8 h. In addition, by the method of RT-PCR, the expression of Rosea1 was detected in the hairy roots treated at 37, 40 and 42 ℃, and a significant correlation between expression pattern and anthocyanin accumulation was found. Therefore, our result suggests that the pBIPhsp::ROS is a useful marker and a tool for the study of the effect of temperature change on gene expression in roots.

Key words: Populus simonii, stable carbon isotope, tree rings, land use type, intrinsic water-use efficiency