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Screening of virulence-attenuated strains for Fusarium oxysporum f. sp. cucumerinum treated by extracts of the rotten root of the parsley.

LI Jie, WANG Yong, GAO Xiao-min, QIAN Cheng, YUN Xing-fu**   

  1. (College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China)
  • Online:2014-10-10 Published:2014-10-10

Abstract: In order to select the virulence-attenuated strains for Fusarium oxysporum f. sp. cucumerinum, the allelopathy and changes of pathogenicity of cucumber plants were detected after treatment by different parsley rotten root extracts on F. oxysporum f. sp. cucumerinum. The F. oxysporum f. sp. cucumerinum were treated from the first generation to fifth generation with parsley rotten roots extracted by ethanol, acetone and aqueous respectively in medium conditions. The results showed that the allelopathy effects of parsley rotten root ethanol extracts of each generation after 144 h treatment were 55.5%, 45.5%, 49.8%, 53.7%, and 44.0% respectively, and the pathogenicity dropped from 43.0% to 4.2% from the first generation to fifth generation. The allelopathy effects of acetone extract treatments were 23.1%, 28.2%, 24.3%, 23.3%, and 19.3% for the first to fifth generation respectively, and the disease index pathogenicity dropped from 35.0% to 3.3%. Both ethanol and acetone treatments had significant difference compared with their corresponding control. The allelopathy effects of aqueous extract treatments were 9.7%, 5.1%, 7.4%, 3.3%, and 2.8% for the first to fifth generation after 144 h treatment, showing no significant difference with their corresponding control, and the disease index dropped from 57.0% to 19.2%. Therefore, it was considered that virulenceattenuated F. oxysporum f. sp. cucumerinum strains could be screened after 5-generation treatment with ethanol or acetone extracts of parsley rotten roots, and they showed a good pathogenicity stability in cucumber plants.

Key words: stable isotopes, heavy metal, food web, Daya Bay, bioaccumulation