Abstract: As an additive, the pollen of transgenic Cry1Ab/Ac insectresistant rice (HH1) was added to the normal feed of Misgurnus anguillicaudatus, which made the artificial feed containing 1%, 5%, 10% and 20% pollen, respectively. M. anguillicaudatus were cultured in laboratory for 100 days fed on one of the artificial feeds mentioned above. Feed with the pollen of nonCry1Ab/Ac parent rice (MH63) added was used as a control, while the regular feed without any pollen was used as a negative control. The ecological safety of transgenic Cry1Ab/Ac rice pollen on M. anguillicaudatus was evaluated by testing the growth performance and activities of physiological enzymes in liver of M. anguillicaudatus. The results showed that no significant differences were found in the weight gain rate, viscera somatic index and liver somatic index of M. anguillicaudatus feeding transgenic Cry1Ab/Ac rice pollen compared to that in the control groups. The activities of two antioxidant protective enzyme, SOD and CAT, and detoxification enzyme GST of M. anguillicaudatus had no significant difference either. With up to 20% pollen content of transgenic Cry1Ab/Ac rice in feed, no obvious influences on the growth performance and physiological metabolism of liver function of M. anguillicaudatus were found after feeding 100 days. The results suggested that the pollen of transgenic Cry1Ab/Ac rice would have no obvious ecological risk on M. anguillicaudatus.

Key words: rectangular flush, maximum chlorophyll fluorescence under illumination, photosynthetic parameters, multiphase flush