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Diversity of culturable actinomycetes in saline-alkali soil in Jiuquan region of Hexi Corridor.

LI Hai-yun1, HU Lei1,2, NIU Shi-quan1**, KONG Wei-bao1, DA Wen-yan1, HAN Cai-hong1, YAN Wei-ru1, GENG Hui1   

  1. (1College of Life Science, Northwest Normal University, Lanzhou 730070, China; 2Institute of Plant Protection and Soil Fertilizer, Hubei Academy of Agricultural Science, Wuhan 430070, China)
  • Online:2015-02-07 Published:2015-02-07

Abstract: In order to reveal the population diversity of culturable actinomycetes in saline-alkali soil in Jiuquan region of Hexi Corridor, Gansu, four soil samples were analyzed using dilution plate coating method, and phylogenetic analysis was conducted based on 16S rRNA gene sequencing. The results showed that total 63 strains of culturable actinomycetes were isolated according to their phenotypes, belonging to 24 genera, 11 families and 7 suborders, among which, 24 strains belonging to genus Streptomyces were the dominant populations, accounting for 38.1% of total number of actinomycetes strains. In addition, there were 22 strains of actinomyces with sequence similarity less than 98% compared with the known strains in GenBank, accounting for 34.9% of total number of actinomycetes strains. These results indicate that actinomycetes resources are abundant with high diversity in the saline-alkali soil in Jiuquan region of Hexi Corridor.

Key words: twig length, leafing intensity, leaf number, twig diameter, leaf area