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Effects of different fertilization regimes on community structure and abundance of soil microorganisms in greenhouse vegetable rhizosphere soils.

WANG Ya-nan1, ZENG Xi-bai1**, WANG Yu-zhong2, BAI Ling-yu1, LI Lian-fang1, WU Cui-xia1, E Sheng-zhe3, MA Shi-ming1   

  1. (1Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agricultural Environment and Climate Change, Ministry of Agriculture of China, Beijing 100081, China; 2Liangzhou Center of Agricultural Technology Extension, Wuwei 733000, Gansu, China; 3Institute of Soil, Fertilizer and Watersaving in Agriculture, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China)
  • Online:2015-03-10 Published:2015-03-10

Abstract: The effects of five fertilization regimes including CK, MNPK, 1/2MNPK, M and NPK on the community structure and abundance of rhizosphere bacteria, ammonia oxidizing bacteria and nirKtype denitrifying bacteria were investigated by the combination of terminal restriction fragment length polymorphism (T-RFLP) and realtime quantitative PCR in longterm greenhouse vegetable soils in Wuwei City, Gansu Province. The results showed that the community structure and abundance of bacteria, ammonia oxidizing bacteria and nirKtype denitrifying bacteria were significantly influenced by both fertilization regimes and rhizosphere effects. Fertilization regimes changed the main populations of the rhizosphere bacteria, ammonia oxidizing bacteria and nirKtype denitrifying bacteria, but their influences were discrepant in different functional microorganisms. The highest abundances of 16S rRNA gene, amoA gene and nirK gene were detected in M, MNPK and M treatments, which were 6.60×109, 1.85×108 and 3.49×107 copy numbers per gram dry soil, and 2.74, 2.81 and 3.46 times of that in CK treatment, respectively. Soil pH, soil organic matter and total phosphorous contents were the key factors influencing the abundances of bacteria, ammonia oxidizing bacteria and nirKtype denitrifying bacteria in greenhouse vegetable rhizosphere soils.

Key words: ammonia oxidizing microorganism, nitrogen cycle, Nitrospira, kinetic theory