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Effects of various treatments on seed germination of Stipa glareosa.

ZHENG Qing-zhong1,2, LI Fa-ming1,2*, ZHU Shu-juan1,2, LIU Shu-juan1,2, LIU Ke-biao1,2, WAN Xiang1,2   

  1. (1Gansu Desert Control Research Institute, Gansu Minqin National Studies Station for Desert Steppe Ecosystem, Lanzhou 730070, China; 2 State Key Laboratory Breeding Base of Desertification and Aralian Sand Disaster Combating, Gansu Desert Control Research Institute, Wuwei 733000, Guansu, China)
  • Online:2016-01-10 Published:2016-01-10

Abstract: This paper reports seed germination rate and seed germination potential  of Stipa glareosa under the treatments of 60 ℃ water soaking, -20/20 ℃ alternating temperature, soaking with 80 mg·L-1 gibberellic acid solution (GA3), drought stress induced by polyethylene glycol (PEG-6000), salt stress induced by Na2CO3 or NaCl solution, the combination treatment of -20/20 ℃ alternating temperature and soaking with 80 mg·L-1 gibberellic acid solution (GA3).  The results showed that: (1) Waterabsorption rate, germination rate and germination potential rate of the seeds soaked in 60 ℃ distilled water for 24 h were 26%, 38% and 32%, respectively; (2) The germination rate and germination potential of seeds treated at -20/20 ℃ alternating temperature for 96 h were 48% and 46%, respectively; (3) After removing of 3/4 of the endosperm, the germination rate, germination potential reached 58% and 54%, respectively; (4) The seed germination rate and germination potential were 54% and 48% under the 24 h long soaking treatment with 80 mg·L-1 GA3 solution. (5) Under the treatment of PEG-6000, the seed germination rate, germination potential, and seed vigor index decreased with the increase of PEG-6000 concentration, and the threshold and maximum PEG-6000 concentrations for germination were 15.9% and 26.3%, respectively. (6) Under the salt stress treatment, the seed germination rate, germination potential, and seed vigor index decreased with the increase of salinity. The decline in seed germination rate under the salt stress treatment of Na2CO3 solution was significantly greater than that of NaCl solution, showing that the inhibition of alkaline salt stress to seed germination is greater than that of neutral salt stress. (7) Under the combination treatment, the seed germination rate and germination potential were 78% and 76%, respectively.

Key words: ozone dry deposition flux, bare soil, soil resistance, eddy covariance