Welcome to Chinese Journal of Ecology! Today is Share:

cje

Previous Articles     Next Articles

Application of high-throughput sequencing for analyzing bacterial diversity in the adductor muscle of sick and healthy Patinopecten yessoensis.

DOU Yan1, ZHAO Xiao-wei1, DING Jun1*, LIU Sheng-cong2, ZHANG Tao2   

  1. (Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, Liaoning, China; Dalian Tianzheng Industry Co., Ltd., Dalian 116023, Liaoning, China).
  • Online:2016-04-10 Published:2016-04-10

Abstract: Healthy and sick samples of Patinopecten yessoensis were taken from different culturing cages. The healthy samples were tagged as CK and the sickness samples were tagged as S1, S2 and S3 respectively. Using MiSeq sequencing method, we compared the microbial structure and variation of P. yessoensis in different healthy status. The results showed that the bacterial diversity was highest in the S3, followed by the CK, S1 and S2. In total, 24 phyla were identified in this study. Among these phyla, Proteobacteria, Bacteroidetes, Planctomycetes, Chloroflexi, Firmicutes, Acidobacteria, Actinobacteria, Nitrospirae, Chlorobi and Fusobacteria took up a large proportion. Proteobacteria were absolutely dominant in the adductor muscle of healthy P. yessoensis, accounting for 95.9% in the entire bacterial community. In the adductor muscle of the sick P. yessoensis, the dominant groups of S1 and S3 were Proteobacteria, accounting for 80.4% and 86.8% respectively, while Bacteroidetes (56.9%) were the dominant phyla in S2.

Key words: Liaoning Province, CO2, energy saving and carbon emission reduction efficiency, undesirable output DEA model