Abstract: Phyllosphere microorganisms play an important role in physiological metabolism and ecological functions of seagrasses. Little is known about the effects of rising temperature on phyllosphere culturable bacteria of Zostera japonica. The abundance, composition, diversity and distribution of phyllosphere culturable bacteria under 26, 28, 30 and 32 ℃ were studied by isolation, counting, RFLP, sequencing and phylogenetic analysis. The abundance of bacteria under different temperatures showed a pattern of 32 ℃>28 ℃>26 ℃>30 ℃. A total of 363 strains were isolated and divided into 24 OTUs. No common OTU was found across all temperatures. All strains derived from α and γproteobacteria, and Marinomonas was the most dominant phyla, which accounted for 86.8% of total abundance under 28 ℃. Pseudomonas and Thalassospira sharply decreased with rising temperature; Marinobacter and Vibrio were enriched under 30 ℃, while Rhodobacteraceae and Ruegeria sharply increased under 32 ℃. The diversity and evenness of phyllosphere culturable bacteria were much higher under 26 and 32 ℃, while the lowest under 28 ℃.

Key words: Salix psammophila community, the Ningxia Habahu National Nature Reserve., Artemisia ordosica community, vegetation stoichiome-try, species diversity