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Effects of three arbuscular mycorrhizal fungi (AMF) species on the growth, physiology, and major components of essential oil of Atractylodes lancea.

LIANG Xue-fei, TANG Meng-jun, LÜ Li-xin, ZHAO Xiang-yu, DAI Chuan-chao*   

  1. (Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China).
  • Online:2018-06-10 Published:2018-06-10

Abstract: To investigate the effects of arbuscular mycorrhizal fungi (AMF) on survival, growth, physiological characteristics, and essential oil accumulation of Atractylodes lancea plantlets, three strains of AMF, including Glomus etunicatum BGC AM0048 (A1), Glomus tortuosum BGC AM0001 (A2), and Glomus mosseae BGC AM0045 (A3), were used to inoculate A. lanceatissue culture seedlings in a greenhouse experiment. The results showed that the inoculation of AMF increased the survival rate of seedlings. The mycorrhizal infection rates in the three inoculated groups were in the order of A3 (63.39%), A2 (47.81%), A1 (42.41%) at 35 d after transplanting, and their positive effects on the growth of A. lancea followed the same order of infection rate, with A3 group showing the best performance. Compared to the control group, plant height, root length, and leaf number per plant in A3 group significantly increased by 21.9%, 65% and 38.7% respectively. The activities of disease resistance enzymes (PAL, PPO, β1,3glucanase), antioxidant enzymes (SOD, CAT and POD) as well as chlorophyll (a+b) content in the leaves of AMF treatment groups were improved at some extent. Moreover, AMF inoculation increased the accumulation of the major components (hinesol, βeudesmol and atractylodin) in the essential oil. Our results showed that there was difference in the preference of AMF for plants. G. mosseae BGC AM0045 (A3) could be used as an excellent fungal strain to inoculate A. lancea seedlings for the establishment of arbuscular mycorrhizae in artificial cultivation.

Key words: regeneration, forest management., seedling and sapling, spatial pattern, natural secondary forest