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Effects of exogenous melatonin on seed germination and physiological indices of rice under As3+ and As5+ stress.

HUANG Yi-zong1*, JIANG Hang1,2, WANG Nong1, LIU Yan1   

  1. (1 Agro-nvironmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China; 2College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China).
  • Online:2018-06-10 Published:2018-06-10

Abstract: We investigated the effects of exogenous melatonin on seed germination and some physiological indices of rice under As3+ and As5+ stress. The results showed that 50 and 100 μmol·L-1 melatonin addition had positive effects on seed germination and growth of rice seedlings under As stress. Melatonin addition significantly increased the activities of antioxidant enzyme, such as CAT, POD, SOD, but decreased MDA content and the rate of O2-· production. At As3+ concentration of 50 μmol·L-1, compared with the control treatment, adding 100 μmol·L-1 melatonin significantly increased the activities of CAT, POD and SOD of rice seedlings by 54.2%, 100.1% and 43.1%, respectively, decreased MDA content and O2-· producing rate by 44.1% and 55.6%. At the same concentration of As5+, adding 100 μmol·L-1 melatonin increased the activities of CAT, POD and SOD of rice seedlings by 62.1%, 49.6% and 47.3%, decreased MDA content and the rate of O2-· production by 33.1% and 34.8%, respectively. The results suggested that the application of melatonin could alleviate lipid peroxidation damage in rice under As3+ and As5+ stress, and effectively alleviate the toxic effects of As3+ and As5+ on rice.

Key words: habitat, Zhangjiajie., Chinese giant salamander (Andrias davidianus), tourism disturbance, water quality