Effect of methanol eluates from Ginkgo biloba root on growth and development of arbuscular mycorrhizal fungi cultured in vitro

Abstract

Abstract: The effect of methanol eluates from Ginkgo biloba root on growth and development of arbuscular mycorrhizal fungi was studied under`cultured in vitro . The study indicated that the methanol eluates from Ginkgo biloba root could significantly stimulate the spore germination and hyphal growth of arbuscular mycorrhizal fungi (Glomus mosseae,Gigaspora margarita),and the 80% methanol eluates had the most significant effect. There were rich flavonoids in the methanol eluates,and the highest flavonoids content was in the 80% methanol eluates. The mathematical analysis showed that there was a significantly positive correlation between the flavonoids content in the methanol eluates and the growth and development of arbuscular mycorrhizal fungi cultured in vitro.

CLC Number:

Q948.3

ZHANG Yong, XIE Liyuan, XIONG Bingquan, ZENG Ming. Effect of methanol eluates from Ginkgo biloba root on growth and development of arbuscular mycorrhizal fungi cultured in vitro[J]. Chinese Journal of Applied Ecology, 2003, (12): 2233-2236.

References

[1] Ai W-D(艾为党),Zhang J-L(张俊伶),Li L(李隆),et al.1999. Phosphorus transfer via mycorrhizal hyphal links from red clover to rye grass.Chin J Appl Ecol(应用生态学报),10(5):615～618 (in Chinese)
[2] Azcon-Aguilar C, Barea JM. 1997. Applying mycorrhiza biotechnology to horticulture: Significance and potentials. SciHort, 68:1～24
[3] Barea JM. 1991. Vesicular-arbuscular mycorrhizaes as modifiers of soil fertility. In: Stewart BA ed. Advances in Siol Science. New York: Springer-Verlag. 1～40
[4] Becard G, Douds DD, Pfeffer PE. 1992. Extensive in vitro hyphal growth of vesicular-arbuscular mycorrhizal fungi in presence of CO₂ and flavonols. Appl Environ Microbiol,58 (3) : 821～825
[5] Becard G, Fortin JA. 1988. New aspects on the acquisition of biotrophic status by vesicular-arbuscular mycorrhizal fungus, Gigaspora margarita. New Phytol, 112:77～83
[6] Carr GR, Hinkley MA, Tacon LF. 1985. Improved hyphal growth of two species of vesicular arbuscular mycorrhizal fungi in the presence of suspension cultured plant cells. New Phytol, 101:417～426
[7] Chen L-Q(陈连庆),Han N-L(韩宁林).1999.Identification of Ginkgo biloba VA mycorrhizalfungiin Zhejiang Province.For Res(林业科学研究),12(6):581 584(in Chinese)
[8] Fery B, Schuepp H. 1993. Transfer of symbiotically fixed nitrogen from berseem (Trifolium alexandrinum L.) to maize via vesicular-arbuscular mycorrhizal hyphae. New Phytol, 122: 447～454
[9] Gianinazzi-Pearson V, Branzanti B, Gianinazzi S. 1989. In vitro enhancement of germination and early hyphal growth of a vesiculararbuscular mycorrhizal fungus by host root exudates and plant flavonoid. Symbiosis, 7: 243～257
[10] Giovannetti M, Citernesi AS. 1993. Time-course of appresorium formation on host plants by arbuscular mycorrhizal fungi. Mycol Res, 97(9): 1140～1142
[11] GongM-Q(弓明钦),ChenY-L(陈应龙),ZhongC-L(仲崇禄).1997. Mycorrhizal Research and Application. Beijing: China Forestry Press. (in Chinese)
[12] Ishii T, Aikawa J, Nakamuta N, et al. 2000. Effect of citrus juice pomace extracts on in vitro hyphal growth of vesicular arbuscular mycorrhizal fungi and their in vitro infections of citrus roots. J Jap Soc Hort Sci, 69(supp.):4～9
[13] Ishii T, Matsumoto I, Shrestha YH. 1995. Axentic culture of vesicular arbuscular mycorrhizal fungi and their infectivity to several plant roots. J Jap Soc Hort Sci, 64(supp): 190～191
[14] Ishii T, Narutaki A, Sawada K, et al. 1997. Growth stimulatory substances for vesicular arbuscular mycorrhiz al fungi in Bahia grass (Pastalum notatum Flugge) root. Plant Soil, 196:301～304
[15] Li X-L(李晓林),Feng G(冯固).2001.Ecology and Physiology of Vesicular Arbuscular Mycorrhiza. Beijing: Huanwen Press.(in Chinese)
[16] Liao L (廖亮). 1994. Study on extracting method total flavones fromGinkgo biloba.FoodSci(食品科学),(8):33～35(in Chinese)
[17] Liou R-J(刘润进),Li X-L(李晓林).2000.Arbuscular Mycorrhizae and Application. Beijing: Science Press. 1～45 (in Chinese)
[18] Liou R-J(刘润进).1996.Theinduction of disease resistance substances of cotton by arbuscular mycorrhizal fungi. Advances in Biological Control of Plant Diseases. Beijing: Agricultural University Press. (in Chinese)
[19] Liu X-Z(刘杏忠),Liu W-M(刘文民),Zhang D-S(张东生).1995. Biological characteristics of Paecilomyces lilacinus associated with soybean cyst-nematode.Chin J Bio Contr(中国生物防治),11(2): 70～74 (in Chinese)
[20] Marcel GA, van der Heijden, John H, et al. 1998. Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature, 396(6706): 69～72
[21] Siqueira JO, Safir GR, Nair MG. 1991. Stimulation of vesicular-arbuscular mycorrhiza formation and growth of white clover by flavonoid compounds. New Phytol, 118:87～93
[22] Suriyapperuma SP, Koske RE. 1995. Attraction of germ tubes and germination of spores of arbuscular mycorrhizal fungus Gigaspora gigantean in the presence of roots of maize exposed to diffent concentrations of phosphorus. Mycologia, 87 : 772～778
[23] Yang X-H (杨晓红). 2001. Study in Growth and Bearing Effect of Arbuscular Mycorrhizal Fungi on Citrus Tree and in Their Traits in vitro Culture (Doctor Dissertation). Chongqing: Southwest Agricultural University. (in Chinese)
[24] Yuan C-Q(袁昌齐). 2001. Exploitation of Natural Medicine Resources. Nanjing: Jiangsu Science and Technology Press. (in Chinese)
[25] Zeng M (曾明). 1999. Study of the Physiological Effect of Arbuscular Mycorrhizae on the Citrus Plants and Their Application (Doctor Dissertation). Chongqing: Southwest Agricultural University.(in Chinese)