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Chinese Journal of Applied Ecology ›› 2003, Vol. ›› Issue (5): 733-736.

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Annalysis of genetic structure of Magnaporthe grisea in the fields of different rice varieties

HE Xiahong, YANG Jing, WANG Yunyue, ZHOU Huiping, CHEN Jianbin, LI Zuosen, LI Yan, ZHU Youyong   

  1. Key Laboratory for Plant Pathology of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China
  • Received:2002-09-06 Revised:2002-12-16 Online:2003-05-15

Abstract: The DNAof 251 Magnaporthe grisea isolates from Shiping County, which originated from monoculture and mixture fields, was extracted and amplified by rep PCRwhich primer was from palindromes Pot2 of Magnaporthe grisea .The result showed that all isolates were amplified 9~17 DNAbands, the size was about from 400bp to 23kb, but mainly between5~10kb. 65% bands were polymorphism bands, and 35% were the same bands. Genetic structures of Magnaporthe grisea in the different rice varieties fields were compared by clustering analysis. The complexions of isolates genetic lineages were related to culture patterns. There were more genetic lineages in mixture field than in monoculture one. The dominant lineages were not distinct in mixture field. The result proved that biodiversity might provide a convincing basis for the steady selection of rice variety to pathogen.

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