Abstract: Experiments were carried out to study the effects of Psources on phosphatase activity of rhizosphere and mycorrhizosphere of red clover inoculated with Glomus mosseae, and cultural system with three compartments was applied.The plants were harvested after 8 weeks,and measured for dry weight and Pcontents as well as soil phosphatase activity of root and hyphal compartment. The results showed that acid phosphatase activity was higher than alkaline phosphatase activity, and both of them were increased slightly after inoculation, especially supplied with organic phosphorus (Na phytate). Acid phosphatase activity was higher than alkaline phosphatase in the mycorrhizosphere of all inoculated treatments.The dry weight, Pcontent and total Puptake of the plants increased significantly after inoculating mycorrhizal fungus.The amount of Puptake by hyphae accounted for 43.1% of total Puptake of plants under appling KH₂PO₄, while the value was 60.8% under appling Na phytate.