Effects of organic acids on the toxicity of cadmium during ryegrass growth

Abstract

Abstract: Effects of low molecular weight organic acids(oxalic acid,citric acid,and acetic acid) and higher molecular weight organic acid(humic acid) on the toxicity of Cd during ryegrass growth were studied.The results showed that Cd toxicity enhanced gradually with increasing the concentration of low molecular weight organic acids,and led to the decreasing of chlorophyll concentration in ryegrass plant and the biomass of ryegrass.The sequence of this influence was:oxalic acid<acetic acid<citric acid.On the contrary,Cd toxicity was reduced as a result of addition of humic acid,and the concentration of chlorophyll in ryegrass shoots and the biomass of ryegrass increased consequently.The concentration of Cd in roots and shoots of the ryegrass increased with increasing the concentration of low molecular weight organic acids,and the sequence of this influence was:citric acid>acetic acid>oxalic acids.The concentration of Cd decreased gradually as a result of increasing the concentration of humic acid,which means humic acid could reduce the toxicity of Cd on ryegrass.Furthermore,the concentration of Cd was higher in roots than in shoots,which indicated that the roots of ryegrass could prevent transport of Cd from roots to shoots and reduce Cd accumulation in the shoots.

CLC Number:

X171.5

LIAO Min, HUANG Changyong . Effects of organic acids on the toxicity of cadmium during ryegrass growth[J]. Chinese Journal of Applied Ecology, 2002, (1): 109-112.

References

[1] Alloway BJ. 1990. Heavy Metals in Soils. Glasgow and London:Blackie.
[2] EPA of China. 1991. Soil Elements Background in China. Beijing:China Environment Science Press. (in Chinese)
[3] Jiao X-Z(焦新之),Feng X-X(冯秀香),Li L(李琳),et al.1980a. Effects of surfactants and 2-4-dinitrophenol on K + transport and salt respiration of excised wheat roots. Acta Phytophysiol Sin (植物生理学报),6(2):191～199(in Chinese)
[4] Jiao X-Z(焦新之),Li L(李琳),Cheng Y-C(程永昌),et al.1980b. The effect of cationic surfactant cetyltrimethyl ammonium chloride on K + uptake by excised wheat roots. Acta Phytophysiol Sin(植物生理学报),6(3):315～322(in Chinese)
[5] Kabata-Pendias A, Pendias H. 1992. Trace Elements in Soils and Plants. Boca Raton: CRC.
[6] Khan KS, Huang CY. 1998. Effects of cadmium, lead and zinc onsize of microbial biornass in red soil. Pedospthhere, 8: 27～ 32
[7] Khan KS, Huang CY. 1998. Effect of acetate on cadmium toxicity to microbial biomass in red soil. J Zgejiang Agric Univ, 24:89～92
[8] Khan S, Khan NN. 1983. Influence of lead and cadmium on the growth and nutrient concentration of tomato Lycopersicum esculentum and egg-plant Solanum melongena. Plant Soil, 74: 387～394
[9] Luo L-X(罗立新),Sun T-H(孙铁珩).1998 Effect of cadmiumsurfactant combined pollution on physiological characteristics of wheat leaf Chin J Appl Ecol(应用生态学报),9(1):95～100(in Chinese)
[10] Mo S-X(莫淑勋)1986. Studies on production and transformation of organic acids in soils and it' s influence on soil properties. Progr Soil Sci(土壤学进展),14(4):1～10(in Chinese)
[11] Piqi AL, Mile RH. 1982. Trans.Min J-K(闵九康),et al 1991.Soil Analysis. China Agricultural Science and Technology Press. (in Chinese)
[12] Sakaki T, Kondo K, Sugahara K. 1983. Breakdown of photosynthetic pigments and lipids in spinach leaves with ozone fumigation:Roles of active oxygen. Physiol Plant, 59: 28～34
[13] Shimazaki K, Sakaki T, Kondo K, et al. 1980. Active oxygen participation in chlorophyll destruction and lipid peroxidation in SO2fumigation leaves of spinach. Plant Cell Physiol, 21: 1193～ 1204
[14] Somashekaraiah BV, Padmaja K, Prasad RK. 1992. Phytotoxicity of cadmium ions on germination seedings of mung bean (Phaseolus vulgarize): Involvement of lipid peroxides in chlorophyll degradation. Physiol Plant, 85( 1 ): 85～89
[15] Stevenson FJ. 1982.Trans.Xia R-J(夏荣基).1994. Humus Chemistry. Beijing: Agricultural University Press. (in Chinese)
[16] Wallace A, Berry WL. 1989. Dose-response curves for zinc , cadmi um and nickel in combinations of one, two or three. Soil Si, 147:401～410
[17] Wang G(王果)1994. Effect of complexation on adsorption of heavy metal ions. Progr Soil Sc (土壤学进展),22(6):6～13(in Chinese)
[18] Wu J-Y(吴家燕),Xia Z-L(夏增禄),Ba Y(巴音),et al 1991.Enzymological diagnoses of soil pollution by heavy metals: Influence of Cd, Cu, Pb and As in purple soil on dehydrogenase of rice roots.Chon J Appl Ecol(应用生态学报),2(1):58～62(in Chinese)
[19] Wu Y-Y(吴燕玉),Wang X(王新),Liang R-L(梁仁禄),et al 1997. Ecological effect of compound pollution of heavy metals in soil-plant system Ⅱ. Effect on element uptake by crops, alfalfa and tree Chom J Appl Ecol(应用生态学报),8(5):545～552(in Chinese)
[20] Yang J-R(杨居荣),He J-Q(何建群),Zhang G-X(张国祥).1996. Reaction of some enzyme activities in crops of different tolerance to the stress of Cd. Chin Environ Sci (中国环境科学),16(2): 113～117(in Chinese)
[21] Yang M-J(杨明杰),Lin X-Y(林咸永),Yang X-E(杨肖娥).1998. Impact of Cd on growth and nutrient accumulation of different plant species. Chin J Appl Ecol (应用生态学报),9(1):89～94 ( in Chinese )
[22] Zhang X-Z(张宪政).1994. Plant Physiology Experimental Technology. Beijing:Higher Education Press.
[23] Zhu B(朱波),Qing C-L(青长乐),Mu S-S(牟树森).1997 Ecological effect of exotic Cd-Zn compound pollution on purple soil.Chin J Appl Ecol(应用生态学报),8(6):639～644(in Chinese)