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Chinese Journal of Applied Ecology ›› 2001, Vol. ›› Issue (5): 757-760.

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Molecular genetic variation of soil bacteria as determined by polymorphism of restrictive fragment length of 16SrRNA genes and its relation w ith vegetation shift

YANG Guanpin, ZHU Yanhong, CHEN Liang, XUE Xiaoqiao   

  1. Faculty of Life Science, Hubei University, Wuhan 430062
  • Received:1999-10-14 Revised:2000-01-14 Online:2001-09-25

Abstract: Bacterial 16S ribosomal RNA (hereafter rDNA) genes were PCR amplified from soil DNA,and cloned without artificial cultivation.The molecular genetic diversity a nd its relation with vegetation shift were determined by RFLP of randomly select ed recombinants containing rDNA inserts.Soils under different vegetation rear re lative bacterial community by formulating their physical and chemical components.The molecular genetic diversity and its differentiation could serve as the appr opriate indicators of vegetation shifts.

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