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Chinese Journal of Applied Ecology ›› 2001, Vol. ›› Issue (5): 777-779.

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Utilization of micronucleus test in Vicia faba root tips cell to detect the mutability of Cu and As pollution

ZHANG Li, LIU Dengyi, WANG Youbao   

  1. College of Life Science, Anhui Normal University, Wuhu 241000
  • Received:2000-12-08 Revised:2001-04-02 Online:2001-09-25

Abstract: The micronucleus test in Vicia faba root tips cell was utilized to detect Cu,As and their combined pollution.The MCN‰ and PI were determined and the F test was used to evaluate the statistical difference in MCN‰ among differente treatments.The results showed that within a certain concentration of Cu2+ (<200mg· L-1) and As3+ (<15 mg·L-1), MCN‰ increased as the concentrat io ns increased.The PI of all the 24 treatment was over 2, and there existed obviou s difference compared with the control (α<0.01abstractThis study shows that Cu, As and combined pollution had significant effects on cytogenetical toxicity of Vicia faba root tip cell.MCN‰ is lower than normal level in the complex treatment of Cu and As because of antagonism actions.

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