Abstract: The change process of ecotone of temperate and subtropical zone and its effect on development, transformation and permeation of chinese agriculture and techniques were discussed in this paper from the viewpoint of landscape ecology. In historical time, the boundary of the ecotone changed northward and southward as climate changed, which changed southward 1～2 latitude in cold period and northward 1～2 latitude in warm period. The ecotone had an obvious environment diversity and strong sensibility, and played important role as a landscape conduit and barrier in aspects of chinese agricultural heterogeneous permeation and heterogeneity creation of agricultural techniques and cultures.