Accumulation of ABA and ODAP in Lathyrus sativus under water stress

Abstract

Abstract: The roots of fifteen days old Lathyrus sativus seedlings were treated by PEG, PEG+ABA and ABA,and their leaves were sampled to determine the contents of endogenic ABA,ODAP,MDA and H₂O₂ and the activity of superoxide dismutase, catalase, peroxidase and glutathione reductase.The results showed that after treated, the contents of ABA and ODAP in leaves increased markedly.The addition of exogenous ABA decreased the increment of MDA and H₂O₂ contents and retarded the attenuation of CAT activity,which were induced by PEG stress,and enhanced the GR activity.In addition,when materials were treated with exogenous ABA in a long time,the ABA content in leaves was increased markedly, and ODAP was then accumulated.There was no significant effect on metabolism of active oxygen in the early days(0～3 days) of ABA treatment,but the activities of SOD,POD,CAT and GR decreased,and the contents of MDA and H₂O₂ increased in leaves of Lathyrus sativus with the duration of ABA treatment(7～15 days).The results indicated that ABA could promote the accumulation of ODAP in leaves of Lathyrus sativus.

XING Gengsheng, Zhou Gongke, LI Zhixiao, CUI Kairong. Accumulation of ABA and ODAP in Lathyrus sativus under water stress[J]. Chinese Journal of Applied Ecology, 2000, (5): 693-698.

References

1 Asada K. 1992. Ascorbate per oxidase-a hydrogen peroxide-scavenging enzyme in plant.Physiol Plant,85: 235～241
2 Britton C, Maehly AC. 1955. Assay of catalase and peroxidase.In:Packer L ed.Methods in Enzymology. Vol Ⅱ.New York:Ac ademic Press Inc.764～775
3 Chandler PM, Robertson M. 1994. Gene expression regulated by abscisic acid and its relation to stress tolerance.Ann u Rev Plant Physiol Plant Mol Biol,45:113～141
4 Colorado P, Rodriguez A, Nicolas G, R odriguez D. 1994. Abscisic acid and stress regulate gene expression during germi nation of chick-pea seeds. Possible role of calcium.Physiol Plant,[ WTHZ 83:457～462
5 Creelman RA, Mason HS, et al.1990. Water deficit and abscisic acid cause differential inhibition of shoot versus r oot growth in soybean seedlings.Plant Physiol,92: 205～214
6 Davies WJ, Zhang J. 1991. Root signal s and the regulation of growth and development of plants in drying soil.Annu R ev Plant Physiol Plant Mol Biol,42: 55～76
7 Department of Chemistry, Lanzhou Univ ersity(兰州大学化学系). 1975. The studies of the analysis of toxin and the metho d of removal toxin in Lathyrus sativus.J Lanzhou Univ(Natural Sciences) (兰州大学学报(自然科学版)),11(2):1～20(in Chinese)
8 Dhindsa RS, Matowe W. 1981. Drought t olerance in two mosses: correlated with enzymatic defense against lipid peroxida tion. J Exp Bot,32: 79～88
9 Dhindsa RS, Plumb-Dhindsa P, Thorpe T A. 1981. Leaf senescence: Correlated with increased levels of membrane permeabil ity and lipid peroxidation, and decreased levels of superoxide dismutase and cat alase.J Exp Bot,32: 93～101
10 Gossett DR, Millhollon EP, Lucas MC. 1994. A ntioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultiv ars of cotton.Crop Sci,34: 706～714
11 Guri A. 1983. Variation in glutathione and a scorbate acid content among selected cultivars of Phaseolus vulgaris prior t o and after exposure to ozone.Can J Plant Sci,63: 733～737
12 Li Z-X(李志孝), Cai W-T(蔡文涛), Xu Z-G(许主国) et al. 1987. The determination of ODAP in Lathyrus sativus using OPT colorimetry.J Lanzhou Univ (Natural Sciences) (兰州大学学报(自然科学版)),[ STHZ 23(2):130～132(in Chinese)
13 Li Z-X(李志孝), Meng Y-F(孟延发), Zhang L(张莉) et al. 1992. Determination of α-and β-oxalyldiaminopropionic acid in Lathyrus sativus by electrophoresis.J Lanzhou Univ (Natural Sciences) (兰州大学学报(自然科学版)),28(3): 89～92(in Chi nese)
14 Liu Z-Q(刘祖祺), Zhang S-C(张石城).1994.Re sistance Physiology. Beijing:Chinese Agricultural Publishing House. 370～372(in Chinese)
15 Malathi K, Padmanaban G, Sarma PS. 1970. Bio synthesis of β-N-oxalyl-L-α,β-diaminopropionic acid, the Lathyrus sati vus neurotoxin.Phytochem,9:1603～1610
16 Moons A, Bauw G, Prinsen E, Montagu MV et al.1995. Molecular and physiological response to abscisic acid and salts in ro ots of salt-sensitive and salt-tolerance indica rice varities.Plant Physiol,107: 177～186
17 Mukherjee SP, Choudhuri MA. 1983. Implicatio n of water stress induced change in the level of endogenous ascorbic acid under hydrogen peroxide in vigna seedlings.Physiol Plant,58: 166～170
18 Pan R-C(潘瑞炽), Dou Z-J(豆志杰), Ye Q-S(叶庆生). 1995. Effect of methyl jasmonic acid on SOD activities and membrane lip id peroxidation in peanut seedlings under water stress.Acta Phytophysiol Sin(植物生理学报),21(30):221～228(in Chinese)
19 Plant AL, Cohen A, Moses MS et al.1991. Nucleotide sequence and spatial expression pattern of a drought- and abscisic a cid-induced gene of tomato.Plant Physiol,97 : 900～906
20 Skriver K, Mundy J. 1990. Gene expression in response to abscisic acid and osmotic stress.Plant Cell,2[ WTBZ : 503～512
21 Wang H-C(王洪春). 1981. Plant resistance ph ysiology.Plant Physiol Commun(植物生理学通讯),6[ WTBZ :72～81(in Chinese)
22 Zeevaart JAD, Creelman RA. 1988. Metabolism and physiology of absicsic acid.Annu Rev Plant Physiol,39:439～473