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Chinese Journal of Applied Ecology ›› 2019, Vol. 30 ›› Issue (6): 2049-2055.doi: 10.13287/j.1001-9332.201906.034

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Effects of enrofloxacin and Cu combined pollution on the activities of digestive enzymes of earthworm in soil.

ZHANG Wei1,2, ZHANG Meng1, CHEN Kai1, JIN Yu-he1, YAN Hui-yi1, HU Yan-mei1, JIN Lan-shu1,*   

  1. 1College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China;
    2Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture/Tianjin Key Laboratory of Agro-Environment and Safe-product, Tianjin 300191, China
  • Received:2018-07-26 Online:2019-06-15 Published:2019-06-15
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (41401573), Ministry of Agriculture Key Laboratory Open Fund (16nybcdhj-5), and Liaoning Province College Students Innovation and Entrepreneurship Training Program (201710157000013)

Abstract: The effects of single and combined pollution of enrofloxacin and Cu on the digestive enzymes of earthworms were studied, based on the actual pollution of caused by the application of livestock feces in farmland soil. Results showed that single enrofloxacin (0.1-4 mg·kg-1, 28 d) did not affect protease, but inhibited cellulase and alkaline phosphatase, with an induced effect on acid phosphatase. Single Cu pollution (20-200 mg·kg-1, 28 d) had inhibitory effects on the four digestive enzymes in earthworms. The effects of combined exposures on the digestive enzymes were mainly negative, showing a synergistic increasing character of toxicity in cellulase and acid phosphatase activities. The response dynamics of digestive enzymes to exposure duration was regulatory response (3 d)-intense response (7 d)-reaction recovery (14 d)-chronic exposure (28 d). Chronic exposure results showed that the combined treatments containing high-dose pollutant (200 mg·kg-1 Cu or 4 mg·kg-1 ENR) had more ecological risk.