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Chinese Journal of Applied Ecology ›› 2019, Vol. 30 ›› Issue (6): 2093-2100.doi: 10.13287/j.1001-9332.201906.027

• Original Articles • Previous Articles     Next Articles

Effectiveness of otolith strontium marking for juvenile Cyprinus carpio.

QIU Chen1,2, JIANG Tao2, CHEN Xiu-bao2, LIU Hong-bo2, YANG Jian1,2,*   

  1. 1College of Wuxi Fisheries, Nanjing Agricultural University, Wuxi 214081, Jiangsu, China;
    2Key Laboratory of Fishery Ecological Environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery, Wuxi 214081, Jiangsu, China
  • Received:2018-08-13 Online:2019-06-15 Published:2019-06-15
  • Supported by:
    This work was supported by Special Financial Project of Ministry of Agriculture of China (CJDC-2017-22), and Central Public-Interest Scientific Institution Basal Research Fund (2016PT01)

Abstract: In this study, we evaluated the effectiveness of SrCl2·6H2O to mark otoliths of juvenile common carp (Cyprinus carpio). The carp fish were immersed with four concentrations of the Sr compound (i.e., 0, 4, 8, 12 mg·L-1) for 2 d to determine the essential concentration for immersing. Then, the carp fish were immersed in 8 mg·L-1 of the Sr compound for five different immersing times (i.e., 1, 2, 3, 4, 5 d) to establish the essential immersing time. Results from electron microprobe analysis (EPMA) showed that otolith Sr marks were very obvious in all C. carpio fish of the aforementioned Sr immersed group with high ratio of Sr/Ca concentration and red color (high Sr level) ring map, compared to the control group with low ratio of Sr/Ca concentration and basic blue color (low Sr level) map. We obtained a 100% mark-success rate of otolith Sr marking in juvenile C. carpio. No significant difference were observed on the average body length, mass and mortality between the immersed group and the control group, suggesting that Sr marking had no negative impact on fish. As clear and complete otolith Sr marked rings occurred at concentration of 8 mg·L-1 or more, and immersing time of 2 d or more for the Sr compound, 8 mg·L-1 and 2 d were suggested as the essential immersing Sr concentration and immersing time, respectively. Our results demonstrated the strong feasibility of otolith Sr marking for juvenile C. carpio.