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    20 November 2018, Volume 29 Issue 11
    Effects of canopy on the redistribution of potassium and sodium ions in rainfall in Quercus acutissima and Camptotheca acuminata mixed plantation of the rainy area of western China
    LIU Yi-lin, WU Fu-zhong, JIANG Long, YOU Cheng-ming, TAN Bo, LI Han-sen, YANG Wan-qin
    2018, 29(11):  3503-3512.  doi:10.13287/j.1001-9332.201811.009
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    The role of canopy in redistributing rainwater K+ and Na+ at different phenological stages (leafless stage, leaf expanding stage, frondent leaf stage and senesced leaf stage) was investigated in Quercus acutissima and Camptotheca acuminata mixed plantation in Mt. Lingyan of Dujiangyan city from December 2016 to November 2017. The concentrations of K+ and Na+ were 1.87 and 1.46 mg·L-1 in the rainfall, respectively, and 5.78 and 1.39 mg·L-1 in the throughfall, respectively. The highest and lowest K+ concentrations in the rainwater were found at leaf expanding stage and frondent leaf stage, respectively. Meanwhile, higher Na+ concentration in rainwater was found at leafless stage and leaf expanding stage, and lower concentration at frondent leaf stage and senesced leaf stage. The inputs of K+ and Na+ through rainfall were 25.47 and 21.60 kg·hm-2·a-1, respectively. The leaching flux of K+ was 13.64 kg·hm-2·a-1 from canopy by rainfall, with the flux of 1.67, 6.23, 2.28 and 3.46 kg·hm-2 at the leafless stage, leaf expanding stage, frondent leaf stage and senesced leaf stage, respectively. On the contrary, the canopy intercepted 11.26 kg Na+·hm-2·a-1, with the interception rate being 32.6%, 18.0%, 44.9% and 31.5% at leafless stage, leaf expanding stage, frondent leaf stage, and senesced leaf stage, respectively. In conclusion, the redistribution of K+ and Na+ in the rainfall affected by Q. acutissima and C. acuminata mixed plantation canopy varied greatly with phenological stages. The results could provide basic data for further understanding of the cycles of K+ and Na+ in the forest ecosystem in the rainy area of western China.
    Changes of non-structural carbohydrates of Pinus sylvestris var. mongolica seedlings in the process of drought-induced mortality
    WANG Kai, SHEN Chao, CAO Peng, SONG Li-ning, YU Guo-qing
    2018, 29(11):  3513-3520.  doi:10.13287/j.1001-9332.201811.005
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    To understand the distribution of non-structural carbohydrates (NSC) and adaptive mechanism in the process of drought-induced mortality, two-year-old Pinus sylvestris var. mongolica seedlings were grown under continuous natural drought condition. Needle water potential and the contents of soluble sugar, starch and total NSC in different organs (current-year-old needles, one-year-old needles, stems, coarse roots and fine roots) of the seedlings were measured when soil water content decreased to 60%, 40%, 30%, 20% and 15% of the soil field water capacity (SFC). The results showed that when the soil water content decreased from 40% SFC to 15% SFC, there was no significant change in needle water potential at predawn and midday. When soil water content decreased from 60% SFC to 30% SFC, the contents of soluble sugar, starch, total NSC and the ratio of soluble sugar and starch first decreased and then increased in all organs. When soil water content dropped from 30% SFC to 20% SFC, the soluble sugar, starch and total NSC contents decreased in the current-year-old needles, one-year-old needles, stems and fine roots. The soluble sugar content increased, but the starch and total NSC contents decreased in the coarse roots. When soil water content decreased from 20% SFC to 15% SFC, the contents of soluble sugar, starch and total NSC decreased in the current-year-old needles, one-year-old needles and stems, and the soluble sugar and total NSC contents decreased, but the starch content increased in the coarse roots, the soluble sugar content decreased, but the starch and total NSC contents increased in fine roots. The results indicated that NSC content in different organs of P. sylvestris var. mongolica seedlings varied in their adaptation to different degrees of drought. The contents of soluble sugar and total NSC in seedlings decreased under less than 30% of the soil field water capacity, with the starch being accumulated in the coarse roots and fine roots. The seedlings might be died due to carbon depletion.
    Relationships between plant community and soil chemical factors in coastal saline area of Shandong, China
    YIN De-jie, ZHANG Jie, JING Rui, DONG Li
    2018, 29(11):  3521-3529.  doi:10.13287/j.1001-9332.201811.007
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    To reveal the correlation between plant communities and soil chemical factors (pH, the total amount of soil soluble salt, TN, TP, SOM, K+, Na+, Ca2+, Mg2+, Cl-, SO42-, HCO3-) in Shandong coastal saline area, a field investigation of 81 samples was carried out and the chemical properties of the soil were analyzed. The results showed that a total of 85 species, 67 genera and 30 families of salt-tolerant plants were recorded. The top three dominant families were Compositae, Gramineae and Chenopodiaceae. The community could be divided into four types: Phragmites aus-tralis - Imperata cylindrical + Artemisia capillaris, Suaeda salsa + P. australis - A. capillaris + Kochia scoparia, P. australis - Typha orientalis + Apocynum venetum, P. australis - A. venetum. The CCA sequencing results showed that the soil total soluble salt and water-soluble ions were the key chemical factors affecting the distribution of salt-tolerant vegetation, followed by total nitrogen content. The community of P. australis - I. cylindrical + A. capillaris had richest species diversity among the four community types, and the resources of salt-tolerant plants were abundant. The species diversity of plant community showed a significant negative correlation with soil total soluble salts and water soluble salt ions content. The plant community diversity index would be reduced by the high saline soil.
    Response of radial growth to climate change in Pinus koraiensis with different diameter classes
    LIU Min, MAO Zi-jun, LI Yue, XIA Zhi-yu
    2018, 29(11):  3530-3540.  doi:10.13287/j.1001-9332.201811.010
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    Dendrochronological techniques were used to study the response of radial growth of Pinus koraiensis with different diameter classes to climate change throughout the natural range of broad-leaved Korean pine forests, including Baishilazi Nature Reserve (40.9° N), Changbai Mountain Nature Reserve (42.4° N), Liangshui Nature Reserve (47.2° N), and Shengshan Nature Reserve (49.4° N). We investigated the similarities and differences of growth responses of different diameter classes to climate factors and clarified the key climate factors affecting the growth of P. koraiensis at different latitude sites. We explored the dynamic changes of the radial growth of P. koraiensis from the latitudinal gradient over the past 40 years. The results showed many similarities in the response of two diameter classes to local climate factors. Small diameter (diameter at breast height of 10-20 cm) trees were more sensitive to the changes of average minimum temperature of the current growing season and the meteorological factors of the previous year, whereas large diameter (diameter at breast height >40 cm) trees were more sensitive to the changes of average maximum temperature and average relative humidity of the current growing season. The key meteorological factors that affecting radial growth of P. koraiensis differed along the latitude gradient. In Baishilazi Nature Reserve, the southernmost plot, the key climate factors were the average maximum temperature and the average maximum temperature in the current growing season. In Shengshan Nature Reserve, the northernmost sample plot, the key climate factors were low temperature factors, such as the minimum temperature in all seasons, the average maximum temperature in winter, the average temperature of all seasons except for the end of previous growing season and the current growing season. The Palmer drought severity index (PDSI) in the current growth season and the end of the current growth season and the precipitation in the current growth season were the key climate factors in Changbai Mountain Nature Reserve. The average temperature of the current growing season was the key climate factor of Liangshui Nature Reserve. In recent four decades, with the rising of temperature, the radial growth of P. koraiensis of two diameter classes significantly decreased in the southernmost point, significantly increased in the northernmost point, and had no significant variation in middle latitudes.
    Self-healing properties of lateral-root branches of three shrub species after fracture force injure in semi-arid mining area
    WANG Bo, LIU Jing, WANG Chen-jia, ZHANG Xin, LIU Jia-wei, LI Qiang, ZHANG Qiang
    2018, 29(11):  3541-3549.  doi:10.13287/j.1001-9332.201811.003
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    To clarify the sustainable soil reinforcement capacity of the lateral root branches of shrubs after injury by fracture force in a semi-arid coal mining subsidence region of Shendong, we analyzed the ultimate anti-fracture mechanical characteristics of three shrub species, Caragana microphylla, Salix cheilophila, and Hippophae rhamnoides, as well as the self-healing ability of their growth indices and mechanical characteristics after injury by fracture force. The results showed that the anti-fracture force and its strength had significant difference among the three shrub species in their late-ral root branches in the early stage of growing season, with the order of C. microphylla > S. cheilophila > H. rhamnoides. The anti-fracture strengths of C. microphylla and S. cheilophila were positively correlated with the contents of cellulose, lignin and holocellulose, while that of H. rhamnoides was significantly negatively correlated with cellulose and lignin contents, but significantly positively correlated with holocellulose content. The fracture force damage caused by activity in the subsidence area significantly reduced the normal growth and mechanical properties of lateral root branches, which could not return to control levels even after three months of self-healing. For the shrubs, stronger self-healing ability of growth indicators resulted in a higher degree of self-healing of anti-fracture forces. Self-healing ability of growth indicators was in the order of H. rhamnoides (91.2%) > S. cheilophila (82.0%) > C. microphylla (73.9%), and self-healing degree of anti-fracture forces was in the order of H. rhamnoides (41.4%) > S. cheilophila (37.1%) > C. microphylla (30.0%). Sustainable soil reinforcement indices of the shrubs’ lateral root branches were in the order of C. microphylla (2.2084) > S. cheilophila (0.2009) > H. rhamnoides (-2.4093). Our results indicated that C. microphylla was the best, S. cheilophila was intermediate, and H. rhamnoides was the least in soil reinforcement in semi-arid coal mining subsidence areas.
    Effects of deficit irrigation on water-radiation use and yield of Coffea arabica under different shade cultivation modes in dry-hot region
    HAO Kun, LIU Xiao-gang, HAN Zhi-hui, YU Ning, CHENG Jin-huan, LIU Chuang, LI Yi-lin, YANG Qi-liang
    2018, 29(11):  3550-3558.  doi:10.13287/j.1001-9332.201811.004
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    The yield and quality of Coffea arabica cannot be guaranteed due to irrational irrigation and light management in dry-hot region. To investigate the best coupling mode of irrigation and shade cultivation of C. arabica in dry-hot region, a field experiment was carried out with different irrigation levels and shade cultivation modes. Three levels of irrigation, i.e., full irrigation, light deficit irrigation and severe deficit irrigation, and four modes of shade cultivation, i.e., no shade cultivation (C. arabica monoculture), light shade cultivation (intercropping of four-line C. arabica and a line banana), moderate shade cultivation (intercropping of three-line C. arabica and a line banana) and severe shade cultivation (intercropping of two-line C. arabica and a line banana) were designed to examine the effects of deficit irrigation on growth, photosynthetic characteristics of lea-ves, water-radiation use and yield of C. arabica under the shade of banana trees. The results showed that net photosynthesis rate (Pn), transpiration rate (Tr), stomatal conductance (gs), leaf water use efficiency (LWUE), leaf radiation use efficiency (LRUE) increased with the increases of irrigation amount, while the intercellular CO2 concentration (Ci) decreased with the increases of irrigation amount. Compared with full irrigation, light deficit irrigation decreased dry bean yield by 9.4%, severe deficit irrigation decreased dry bean yield and water use efficiency (WUE) by 36.7% and 16.9%, respectively. Pn, Tr, gs, LWUE first increased and then decreased with the increases of shading degree, with moderate shade cultivation having the maximum increment. Compared with no shade cultivation, light shade cultivation increased dry bean yield and WUE by 13.0% and 12.9%, respectively; moderate shade cultivation increased dry bean yield and WUE by 23.1% and 23.4%, respectively. The dry bean yield, WUE, volume and fresh mass of 100-bean of C. arabica increased in different degrees with the increases of irrigation amount and shading degree, with the largest enhancement of dry bean yield and WUE occurring in full irrigation under moderate shade cultivation. Soil water content in the same soil depth decreased with the increases of shading degree. Soil water content in the same place increased first and then decreased with the increases of soil la-yer depth in 0-50 cm soil layer. Results of regression analysis showed that LRUE had a significantly negative exponential relation or Logistic-curve variation with photosynthetically active radiation. Therefore, the suitable combination of irrigation treatment and banana shade cultivation mode for C. arabica was full irrigation and moderate shade cultivation, considering the comprehensive benefit of superior quality and high production and high efficiency water-radiation use.
    Composition and niche of the existing herbaceous plants in the water-level-fluctuating zone of the Three Gorges Reservoir Area, China
    GUO Yan, YANG Shao, SHEN Ya-fei, XIAO Wen-fa, CHENG Rui-mei
    2018, 29(11):  3559-3568.  doi:10.13287/j.1001-9332.201811.006
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    To understand the usages of available resource by dominant plants, their niches and the mechanisms of inter-specific competition and co-existence in the water level fluctuation zone, we studied the spatial distributions and niche characteristics of existing dominant herbaceous species at a typical water-level-fluctuation site of the Three Gorges Reservoir Area, Zigui. The results showed that there were 39 herb species in total, which belonged to 18 families and 32 genera. Gramineae, Compositae, Polygonaceae and Euphorbiaceae were the dominant families. Cynodon dactylon, Setaria viridis, Bidentis tripartitae and Digitaria chrysoblephara were the dominant species, with high importance value and niche breadth. In addition, at 145-155 m, 155-165 m and 165-175 m altitude section, the three major species, expressing the highest ecological niche breadth, which were in order of C. dactylonPolygonum lapathifoliumS. viridis, S. viridisD. chrysoblepharaC. dactylon, and S. viridisB. tripartitaeP. orientale, respectively. The niche overlap of the species between the different altitudes zone was relatively high. The species which had a broad niche could co-exist with those occupying narrow niche. The niche overlap could not be determined by niche breadth lonely. Furthermore, after seven times of water level fluctuations, most of the species were annual herbs, and the degree of niche differentiation was low in the area. Due to scarce resources and unstable habitats, the inter-specific competition was strong, and the vegetation was at the primary successional stage.
    Physiological integration of growth, carbohydrates, and soluble protein of Zoysia japonica clonal ramets under nutrient heterogeneity
    XU Su-nan, LIU Yan-hong, LI Hong-yi, CHEN Zhong-lin, LI Yue, ZHANG Li-hong
    2018, 29(11):  3569-3576.  doi:10.13287/j.1001-9332.201811.002
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    This study was carried out to analyze the changes of growth, the contents of carbohydrates and soluble protein of Zoysia japonica clonal ramets under nutrient heterogeneity, where the connected and disconnected ramets were treated with different nutrient levels. The results indicated that under the nutrient heterogeneity the parent ramets in middle or high nutrient levels improved the aboveground biomass, belowground biomass, and total biomass, with the enhancement of 32.5%, 22.1% and 24.8% at high nutrient level, respectively, reduced the root/shoot ratio, the contents of soluble sugar and non-structural carbohydrates (NSC), with the reduction of 7.7%, 15.2% and 13.1% in high nutrient level, respectively, but had no significant impacts on the contents of starch, cellulous, and soluble protein of the connected daughter ramets. The daughter ramets in middle and high nutrient levels had no significant impacts on the growth and the contents of carbohydrates and soluble protein of the connected parent ramets. There was a significant physiological integration effects from parent to the daughter ramets on the biomass, root/shoot, the contents of soluble sugar and NSC. The intensity of physiological integration was proportional to the nutrient gradient of ramets, but had no significant physiological integration effects on the contents of starch, cellulous and soluble protein. The daughter ramets had no physiological integration for any indicator of the parent ramets. There was a unidirectional physiological integration between parent and daughter ramets of Z. japonica.
    Spatial heterogeneity of soil water content under introduced shrub (Caragana korshinskii) in desert grassland of the eastern Ningxia, China
    ZHAO Ya-nan, ZHOU Yu-rong, WANG Hong-mei
    2018, 29(11):  3577-3586.  doi:10.13287/j.1001-9332.201811.001
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    Combined with geographic information system (GIS) data, the spatial heterogeneity of soil water in the 0-200 cm soil layer of the desert steppe with the introduction of the shrub Caragana korshinskii in eastern Ningxia was studied by classical statistical and geostatistical methods. It was found that the soil water content of each soil layer in the 0-200 cm profile was relatively low, ranging from 0.6% to 19.0% (averaged 4.4%). The coefficient of soil water content variation ranged from 49.5% to 86.3%, which indicated moderate spatial heterogeneity. The variation of soil water content at the 0-60 cm and 80-120 cm soil layers fitted the spherical model, while that at the 60-80 cm soil layer fitted the exponential model and that at the 120-200 cm soil layer fitted the Gaussian model. Soil water content at different soil layers showed different degrees of spatial autocorrelation. The nugget/sill [C0/(C0+C)] of soil water content in the 0-40, 60-80, and 120-200 cm soil layers ranged from 26.1% to 49.9%, with moderate spatial correlations. The nugget/sill of soil water content in the 40-60 cm, 80-100 cm, and 100-120 cm soil layers ranged from 15.5% to 22.1%, with strong spatial correlations. The scale of spatial heterogeneity in soil water content (A0) varied among soil layers. The A0 was relatively large in the 0-20 and 20-40 cm soil layers (37.10-45.18 km) and small in the 40-200 cm soil layers (3.58-8.66 km). The results indicated that the introduction of C. korshinskii shrubs into desert grassland for vegetation recovery in recent decades have depleted soil water and accelerated the depletion of soil water in the deep layer (40-200 cm), leading to spatial heterogeneity and fragmentation of soil water (0-200 cm) with stronger effects at deep soil layer.
    Prezygotic reproductive isolation of two sympatric species of Ligularia weeds in eastern Qinghai-Tibet Plateau, China
    WANG Yan-ning, WANG Bo-sen, HOU Qin-zheng, SU Xue, ZHANG Shi-hu, SUN Kun
    2018, 29(11):  3587-3595.  doi:10.13287/j.1001-9332.201811.008
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    Reproductive isolation, the key process to prevent interspecific hybridization and keep the completeness and independence of species, is crucial to the formation and maintenance of biodiversity. The forming steps, methods and intensities of reproductive isolation between different species are not completely the same. Literatures on reproductive isolation between species provide valuable evidence for speciation and its maintenance. Flowering phenology, flowering-visiting insects, and pollen-stigma compatibility were observed for Ligularia virgaurea and L. sagitta, two species of weeds sympatrically distributed in eastern Qinghai-Tibet Plateau. The characteristics of reproductive isolation mechanism of both species and its role in reproductive isolation were also studied. The results showed that the flowering period of L. virgaurea was 20 d earlier than that of L. sagitta. Although the full-blooming period separated, there was a roughly 10 d overlap between the two species. Meanwhile, the pollens and stigmas of the two species were both viability in the overlapped 10 d. L. virgaurea and L. sagitta shared the same types of pollinating insects, which was a generalized pollination type. Besides, the flower-visiting behavior towards the two species from the pollinators was similar, along with the interleaved access between the two species. There was no complete time isolation and no complete pollinator isolation between the two species, but the stigma had poor compatibility towards heterogenic pollen. The germination of pollen on stigma and growth of the pollen tube was hampered. Furthermore, the rejection of pollen-stigma was likely to be an important prezygotic reproductive isolation between L. virgaurea and L. sagitta, thus avoiding the natural hybridization and maintaining the diversity and genetic stability of the two species.
    Photosynthetic mechanism of tea yield and quality affected by different habitats
    WANG Ye, ZHANG Guo-lin, YANG Shu-ying, ZOU Dong-sheng, XIAO Run-lin, ZHU Han-yang, WANG Hao
    2018, 29(11):  3596-3606.  doi:10.13287/j.1001-9332.201811.029
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    To understand photosynthetic mechanism of tea yield and quality, an experiment was conducted with four different typical habitats, including three intercropping patterns (S1:Osmanthus-Tea, S2:Michelia-Tea, S3:Osmanthus-Michelia-Tea) and a control (CK) at Changsha Agricutural Observation Station of Chinese Academy of Sciences. The photosynthetic physiological and ecological characteristics of tea yield and quality were examined. The results showed that the habitats S1, S2, S3 reduced the leaf temperature (TL), photosynthesis active radiation flux (PAR), net photosynthetic rate (Pn), transpiration rate (Tr), and stomatal conductance (gs), as well as the tea polyphenol content. Habitats S1, S2, S3 significantly increased leaf relative humidity (RHS), total amino-acid content of tea, and the yield and quality of tea, with a pattern of S3>S1>S2>CK. The leaves in habitats S1 and S3 could be made into high-grade green tea and famous green tea respectively. Comprehensive indicators showed that habitat S3 is an ideal intercropping pattern for high quality and high yield of tea garden.
    Ecological and biological effects of biodegradable film on cotton in Hexi area of Gansu, Northwest China
    WANG Ning, FENG Ke-yun, NAN Hong-yu, LI Ya-bing
    2018, 29(11):  3607-3614.  doi:10.13287/j.1001-9332.201811.026
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    To examine the effects of biodegradable film on preservation of soil temperature, moisture, growth and yield of cotton fields in Hexi area of Gansu, the trials were conducted with four treatments of DA (biodegradable film A with thickness of 0.012 mm), DB (biodegradable film B with thickness of 0.008 mm), common plastic film and unmulching were used to analyze the changes of degradation property, soil temperature, moisture, weeds control, and yield in 2016 and 2017. The results showed that the induction period of DB was three to five days earlier than that of the DA and the degradation rate was higher than DA. The mass loss rate of DA and DB could reach 95.6% and 94.5% respectively under 180 days landfill treatment. The DA showed good performance in water retention, soil warming in seedling stage, with no significant difference with the common plastic film. Due to the high degradation rate, water retention property, and insulation of DB were significantly lower than that of common plastic film. The growth period of cotton prolonged by biodegradable film compared with common plastic film, but with no significant difference of the emergence rate, boll number per plant, boll mass and lint percentage. However, the pre-frost yield and weeds control effects significantly decreased. There was no significant difference of cotton yield of DA compared with common film, but significantly increased by 73.1% and 59.9% compared with unmulching. The cotton yield of DB was significantly decreased by 11.8% and 7.1% compared with common film. In summary, DA played good performance on the preservation of soil temperature, moisture and the yield increasing effect, which could be applied in Hexi agricultural area of Gansu.
    Differences in light and heat utilization efficiency and yield of soybean in two ecological zones and their response to chemical control regulators
    WANG Chang, ZHAO Hai-dong, FENG Nai-jie, ZHENG Dian-feng, LIANG Xiao-yan, QI De-qiang, HUANG Wen-ting
    2018, 29(11):  3615-3624.  doi:10.13287/j.1001-9332.201811.021
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    The field experiment was conducted at two farms at Jiusan in Heihe (the fourth accumulated temperature zone) and at Lindian County of Daqing (the second accumulated temperature zone), both sites located in Heilongjiang Province, China. With soybean Kenfeng 41 as the test material, uniconazole (S3307, 50 mg·L-1) and 2-N, N- diethylamino ethyl caproate (DTA-6, 50 mg·L-1) were sprayed on leaves in the early flowering period of soybean. Through grey correlation analysis, the main factors affecting soybean yield were examined, and the differences of the light and heat utilization efficiency and soybean yield in two ecological conditions were compared. The regulation effects of chemical control technology on the light and heat utilization efficiency of soybean were explored. The results showed that the total surface radiation and ≥10 ℃ effective accumulated temperature were the main factors affecting soybean yield in both areas compared with rainfall and sunshine hours. The light and heat utilization efficiency from sowing to flowering period was significantly positively correlated with dry matter accumulation, and that from flowering to podding period was significantly positively correlated with dry matter accumulation per plant. There was a significant positive correlation between yield and dry matter accumulation, grain number per plant, grain mass per plant and 100-grain mass at seedling stage to podding stage. S3307 and DTA-6 could significantly improve light and heat utilization efficiency and soybean yield in both areas. S3307 showed the better regulation function to impact the light and heat utilization efficiency and yield than DTA-6 in both sites. In the two ecological areas of Jiusan and Lindian, spraying S3307 increased light utilization efficiency by 13.6% and 17.1%, and increased heat utilization efficiency by 14.1% and 17.2%, respectively. The yield by spraying S3307 was increased by 14.1% and 17.3% separately in Jiusan and Lindian. Therefore, it is the effective way to enhance resources utilization and achieve high-yield by using the reasonable chemical control technology.
    Effects of micro-sprinkling hose irrigation with different hose lengths on soil water distribution, dry matter accumulation, and grain yield of wheat fields
    WANG Bing-xin, ZHAO Jun-ye, SHI Yu, YU Zhen-wen
    2018, 29(11):  3625-3633.  doi:10.13287/j.1001-9332.201811.025
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    To explore the optimal hose length of micro-sprinkling hose irrigation in wheat fields, a field trial taking JiMai 22 as test material was carried out in two growing seasons (2015-2016 and 2016-2017). Three lengths of micro-sprinkling hoses with 80-mm width were used, including 60 m (T1), 80 m (T2) and 100 m (T3). The length of trial plot was equal to the hose length. The trial plots were divided to different sample sections every 20-m length along the irrigation direction, which were named as A, B, C, D and E sections, respectively, to examine the effects of micro-sprinkling hose irrigations with different hose lengths on soil water distribution, dry matter accumulation and grain yield of wheat fields. The results showed that: 1) After irrigation at the jointing and anthesis stages in the two growing seasons, the relative soil water content in the 0-40 cm soil layer showed T1<T2<T3 in the A section, T1, T2<T3 in the B section, T1>T2, T3 in the C section and T2>T3 in the D section. The CV of relative soil water content in different sections in the same treatment showed T1<T2<T3. 2) Leaf area index and the rate of canopy photosynthesis active radiation interception at 20 d and 30 d after anthesis, and dry matter accumulation amount after anthesis and at the maturity stage had no significant differences in the A or B section among different treatments. Those indices showed T1>T2, T3 in the C section and T2>T3 in the D section. Leaf area index and rate of canopy photosynthesis active radiation interception at 20 d and 30 d after anthesis and dry matter accumulation amount after anthesis showed T1, T2>T3, and dry matter accumulation amount at the maturity stage showed T1> T2>T3. 3) In the two growing seasons, grain yield in the A and B sections had no significant differences among different treatments, and that showed T1>T2, T3 in the C section and T2>T3 in the D section. Grain yield of each treatment showed T1, T2>T3. 4) The grain yield and water use efficiency showed T1> T2>T3, and the irrigation water use efficiency showed T1>T2>T3 among different treatments in the two growing seasons. Considering grain yield and water use efficiency, hose irrigation with micro-sprinkling hose at 80-mm width and 60-m length was optimal treatment for water-saving and high-yield irrigation, and the suboptimal length was 80 m under this condition. The results could provide theoretical basis for water-saving and high-yield irrigation with micro-sprinkling hose in wheat fields in Shandong Province.
    Effects of irrigation regimes on the variation of soil water and salt and yield of mechanically harvested cotton in Southern Xinjiang, China
    CUI Yong-sheng, WANG Feng, SUN Jing-sheng, HAN Qi-sheng, WANG Jing-lei, LI Nan
    2018, 29(11):  3634-3642.  doi:10.13287/j.1001-9332.201811.028
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    The expansion of soil salinization area is a threat to cotton production in Southern Xinjiang. How to control soil salt accumulation by developing precise irrigation strategy is currently a hot topic for the film mulching drip-irrigated cotton field. With aims for soil quality improvement and high yield, we investigated the effects of irrigation regimes on soil water and salt distribution, yield and fiber quality in major cotton production area of Southern Xinjiang. Results showed that the increase of irrigation amount contributed to a higher cotton photosynthetic product accumulation, but had no significant effect on the ratio of reproductive organs to total biomass. The degree of soil desa-lination had a positive correlation with the irrigation quotas. When the seasonal total irrigation amount exceeded 2577.83 m3·hm-2, soil salinization would not deteriorate. Irrigation amount had significant effect on WUEI, but had no effect on fiber quality. With the increasing of irrigation amount, cotton yield increased first and then decreased. In conclusion, applying 4200 m3·hm-2 seasonal irrigation amount in total and 7 d and 5 d irrigation interval for the budding and flowering stage respectively could be a suitable irrigation regime for mechanically-harvested cotton in the arid area of Southern Xinjiang
    Effects of straw returning on maize yield and root system spatial distribution under water stress
    WANG Feng, WANG Mei-jia, SU Si-hui, WANG Ying-yan, SU Ye-han, MENG Guang-xin, SUN Yue, QI Hua, JIANG Ying
    2018, 29(11):  3643-3648.  doi:10.13287/j.1001-9332.201811.023
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    To investigate the effects of straw amendments on the yield and root spatial distribution of maize under water stress, an experiment with rainproof shelter was conducted in the field experimental station of Shenyang Agricultural University in 2016 and 2017. The drip irrigation facilities were used to perform water stress treatments. Straw burying (T1) and straw incorporation (T2) as two approaches of straw amendments were conducted combined with three depths of 15 cm (D1), 30 cm (D2), and 45 cm (D3) for straw returning, ploughing tillage at above three depths without straw presence as control in this study. During seedling and silking stages of maize, drought and water logging stresses were introduced respectively to the plants. Our results showed that the yield of maize under S1T1D2 treatment in 2016 was significantly increased by 5.7%-7.1%. Compared with all the rest treatments, the dry weights of lateral roots and deep roots under S1T1D2 treatment were increased by 67.3%-149.9% and 17.9%-116.4%, respectively. The dry matter accumulation in shoot of maize observed from S1T1D2 treatment was significantly lower than those under other treatments, with 2.1%-35.8% reduction. Our results indicated that S1T1D2 could significantly promote the growth and spatial distribution of maize root, accounting to release water stress and keep yield stabilization or promotion. Therefore, 30 cm of straw burying could be used as a suitable approach of straw returning for maize production in northeastern China, where the climate is with a pattern of drought first and waterlogging in later stage.
    Effects of light quality ratio on photosynthetic characteristics and quality of purple lettuce
    GAO Yong, LI Qing-ming, LIU Bin-bin, LI Shu-hao, AI Xi-zhen, WEI Min, ZHANG Da-long
    2018, 29(11):  3649-3657.  doi:10.13287/j.1001-9332.201811.024
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    To explore the effects of different light quality ratios on the growth and development of purple lettuce, we used LED intelligent light control console as light source to study the effects of adding different proportions of red and blue light (1:1, 2:1, 4:1 and 1:2) to the white light on photosynthetic characteristics and commercial quality of purple lettuce (Lactuca sativa L. cv ‘Zhongshu Purple Lettuce’). The results showed that when the ratio of red to blue light was 4:1, the chlorophyll content, RuBP carboxylase activity, electron transport efficiency of photosynthesis, net photosynthetic rate and growth were all significantly higher than that in control and other treatments, whereas the nitrate content was the lowest. When the ratio of red to blue light was 1:2, the contents of soluble protein, vitamin C, secondary metabolites such as anthocyanins, flavonoids, total phenolic compounds, and the contents of antioxidant capacity were significantly higher than those in the control and other treatments. We concluded that adding appropriate ratios of red to blue light to the white light could increase photosynthesis and improve commercial quality of purple lettuce.
    Emergy analysis, water-heat utilization, and carbon emission of typical cropping patterns in the oasis irrigation area.
    YIN Wen, CHAI Qiang, FAN Zhi-long, HU Fa-long, ZHAO Cai, YU Ai-zhong
    2018, 29(11):  3658-3668.  doi:10.13287/j.1001-9332.201811.022
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    Reasonable cropping pattern can improve resource utilization efficiency, reduce environmental risks in agricultural production, and achieve the goal of resource saving coupled with high production and efficiency. We evaluated the production effects of typical cropping patterns in an arid irrigation region from several aspects, including resource utilization, carbon emissions, economic benefit, emergy self-sufficiency ratio and net emergy yield ratio, with the method of emergy theory, to provide theoretical and practical basis for the establishment of efficient cropping with lower resource investment and carbon emissions but higher yield and resource utilization efficiency combined with the sustainability of agricultural production. The results showed that, high-efficient wheat-maize intercropping production pattern (integration of no tillage with 25 to 30 cm height of wheat straw covering in the wheat strip and two-year plastic mulching in the maize strip, NTSI) had the best performance in grain yield boosting, with the yields 13.5% to 16.9% and 13.8% to 17.1% higher than that in local traditional production patterns (i.e., wheat-maize intercropping, CTI, and monoculture maize, CTM), respectively. Moreover, NTSI increased water use efficiency and solar energy use efficiency by 12.4% to 17.2% and 6.1% to 8.1%, compared to CTI, respectively. Compared to CTI and CTM, NTSI improved the effect on carbon emission reduction by decreasing total soil CO2 emission by 618 to 895 kg·hm-2 and 1804 to 2002 kg·hm-2 with the ratio of 12.1% to 16.4% and 28.6% to 31.0%, but increased carbon emission efficiency by 29.3% to 40.1% and 58.9 to 71.4%, respectively. Meanwhile, the NTSI pattern had obvious resources saving potential. Compared with CTI and CTM, NTSI reduced total input by 1424 to 1431 yuan·hm-2 and 501 to 1547 yuan·hm-2, with the proportional reduction being 12.6% to 13.6% and 4.9% to 14.6%; however, increased total output by 4309 to 4603 yuan·hm-2 and 8439 to 11057 yuan·hm-2, the increased ratio was 11.2% to 11.8% and 24.4% to 36.3%; also, increased net return by 5740 to 6027 yuan·hm-2 and 9544 to 11558 yuan·hm-2, and the improved percentage was 19.6% to 22.4% and 40.1% to 57.7%, respectively. Therefore, the high-efficient NTSI pattern had greater input-output ratio by 27.9% to 29.0% and 40.5% to 45.6% than CTI and CTM, respectively, similarly, greater benefit per cubic meter of water by 19.9% to 23.2% and 27.7% to 39.3%, respectively. The emergy self-sufficiency ratio of NTSI pattern was 57.2%, being 4.0% and 12.2% higher than CTI and CTM, respectively. NTSI pattern’s net emergy yield ratio was 0.173, being 10.0% greater than CTI but 11.7% lower than CTM. With respect to resource utilization, carbon emission reduction, economic benefits and the sustainable development of high-efficient intercropping pattern, the integration of no tillage with straw covering and two-year plastic film mulching measures had higher economic activity, better function of agricultural system, greater production efficiency, and higher returns on energy value. We concluded that NTSI could be a high-efficiency farming system pattern on resources saving, carbon emission reduction, high yield and efficiency in arid oasis irrigation areas.
    Dynamic of carbon footprint and its composition for double rice production in Southern China during 2004-2014
    CHEN Zhong-du, XU Chun-chun, JI Long, FANG Fu-ping, CHEN Fu
    2018, 29(11):  3669-3676.  doi:10.13287/j.1001-9332.201811.027
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    The area of rice production in Southern China is one of the most important farming regions in China, due to its great contribution to national food security. To assess the differences in carbon footprints (CF) of double rice production will benefit the achievement of low carbon agriculture. Base on the statistical data of crop yield and farmland investment of double rice production from 2004 to 2014, the spatiotemporal dynamics of CF and its composition of double rice in Southern China was estimated using the theory of CF and life cycle assessment method. The results showed that the CF of early and late rice in each province tended to increase, with the CF of early rice being higher than that of late rice. CF of early and late rice in Anhui were the highest (1000 kg CO2-eq·hm-2), and those in Fujian, Hubei and Hunan were the lowest (750 kg CO2-eq·hm-2). The highest component of CF in double rice was the production, transportation and utilization of fertilizers (60%), followed with the consumption of diesel. Progressive regression analysis showed that CF of double rice from 2004 to 2014 was mainly affected by diesel, compound fertilizer and K2O fertilizer. The analysis of net profit income showed that Hubei was a province with low-emission and high-yield, which was conducive to the sustainable development of low carbon agriculture. Given to the large transfer of rural labor from agricultural to non-agricultural sections and the development of mechanization, diesel and other mechanical inputs would increase rapidly in the future. Therefore, improving fertilizer utilization, mechanical and irrigation efficiencies in crop production would be the main approach to promote low-carbon agriculture in rice production of southern China.
    Radical variation of main wood properties and its relationship to climatic factors of Machilus pauhoi plantation
    YI Min, LAI Meng, ZHANG Lu, CHEN Fu-shen, HU Song-zhu
    2018, 29(11):  3677-3684.  doi:10.13287/j.1001-9332.201811.014
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    To provide a theoretical foundation for forecasting wood properties, rotation selection and efficient use of timber, wood properties of 17 year-old Machilus pauhoi plantation were examined using SilviScanTM fast detection technology. The radical variation mode of anatomical traits, wood density, modulus of elasticity and their responses to climate change were analyzed. We also analyzed the relationship between growth ring width, growth ring age and wood property parameters. The results showed that the radical variation patterns of all the wood properties were different, with 7-11th growth ring being the turning point of the variation curves. Growth ring width had no significant correlation with wood density and cell wall to cavity ratio. Growth rate and growth ring age imposed significant impacts on most wood properties. Different climatic factors had different impacts on different wood properties. Relative humidity was the main factor affecting wood properties. The responses of microfibril angle and modulus of elasticity to climate change were more sensitive than other wood properties.
    Additive aboveground biomass equations based on different predictors for natural Tilia Linn
    WANG Jia-hui, LI Feng-ri, DONG Li-hu
    2018, 29(11):  3685-3695.  doi:10.13287/j.1001-9332.201811.020
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    Biomass is a basic quantitative character of forest ecosystem. Biomass data are foundation of researching many forestry and ecology problems. Accurate quantification of tree biomass is critical and essential for calculating carbon storage, as well as for studying climate change, forest health, forest productivity, nutrient cycling, etc. Constructing biomass models is considered a good approach to estimate forest biomass. Based on biomass data of 97 sampling trees of natural Tilia Linn. in Xiaoxing’an Mountains and Zhangguangcai ranges, three additive systems of individual tree biomass equations were developed: based on tree diameter at breast height (D) only, based on tree diameter at breast height and height (H), and based on the best models. The nonlinear seemly unrelated regression was used to estimate the parameters in the additive system of biomass equations. The heteroscedasticity in model residuals was addressed by applying a unique weight function to each equation. The individual tree biomass model validation was accomplished by Jackknifing technique. The results showed that three additive systems of individual tree biomass equations could fit and predict the biomass of Tilia Linn. well (adjusted coefficient of determination Ra2>0.84, mean predicted error percentage MPE<8.5%, mean absolute error MAE<16.3 kg,mean standard error percentage MPSE<28.5%). The biomass equations of stem and aboveground were better than biomass equations of branch, foliage and crown. Adding total tree height and crown factor in the additive systems of biomass equations could significantly improve model fitting performance and predicting precision (Ra2 improved from 0.01 to 0.04, MAE decreased from 0.01 to 4.55 kg), narrow the confidence interval of the predicted value and the biomass of stem, foliage and aboveground were increased more than the biomass of branch and crown. In general, the equations of the additive system based on the best models produced the best model fitting, followed by that of the additive system based on D and H, and that based on D. It was essential to develop biomass model by adding total tree height and crown factor.
    Potential geographical distribution of Pyrus calleryana under different climate change scena-rios based on the MaxEnt model
    LIU Chao, HUO Hong-liang, TIAN Lu-ming, DONG Xing-guang, QI Dan, ZHANG Ying, XU Jia-yu, CAO Yu-fen
    2018, 29(11):  3696-3704.  doi:10.13287/j.1001-9332.201811.016
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    To resovle the problems of in-situ conservation and resource utilization of Pyrus caller-yana, maximum entropy model (MaxEnt) and geographic information system (GIS) were used to predict the global ecological suitable region of P. calleryana in different climate scenarios based on 236 distribution data and 19 ecological factors. The results showed that the ecological suitable regions of P. calleryana were mainly concentrated in North America, Asia and other regions, with a total area of 1.6×107 km2. In China, the regions with high ecological suitability were Hunan, Hubei, Anhui, Jiangxi, Jiangsu, Zhejiang and Fujian provinces. The main factors affecting the geographical distribution of P. calleryana were mean annual temperature and mean annual precipitation, followed by the seasonality of temperature. The model simulations indicated that P. calleryana would have different suitable habitat areas and marginally suitable habitat areas in different climate scenarios. In terms of the spatial distribution of the potential habitat area, both the distributional range and the center of distribution of suitable and marginally suitable habitat area would shift from east to west. The suitable habitat area in North America and marginally suitable habitat areas in Europe would increase rapidly.
    Hydrological effects of vegetation restoration and the threshold for its response to annual precipitation in Pengchongjian small watershed, Jiangxi, China
    LIU Zheng, YE Jing-ping, OUYANG Lei, SHENG Fei, LIU Shi-yu
    2018, 29(11):  3705-3711.  doi:10.13287/j.1001-9332.201811.017
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    To examine the change of runoff and its reason is an important scientific issue in forest hydrology. In this study, we performed the trend and inflection analysis on the time series of daily precipitation and runoff in the Pengchongjian small watershed from 1983 to 2014 by Mann-Kendall test method, a site with abundant precipitation . Using the empirical statistics method, we analyzed the effects of precipitation variation and vegetation restoration on the runoff and its contribution rates. Furthermore, we calculated the critical value of the hydrological effect of vegetation restoration on the annual precipitation. The results showed that the year 2003 was a consistent abrupt point for annual precipitation and runoff. Compared to the baseline period (1983-2003), annual precipitation and the depth of runoff in the changing period (2004-2014) decreased by 8.7% and 29.2%, with the averaged annual decrease of 12.7 and 22.1 mm, respectively. The averaged depth of runoff in spring, summer, autumn, winter, and in the whole year decreased by 100.2, 105.8, 25.2, 23.4, and 243.0 mm, respectively. The contribution rates of the depth of runoff to precipitation varia-tion were 58.9%, 71.6%, 65.5%, 35.0%, and 57.1%, respectively, while the contribution rates of vegetation restoration were 41.1%, 28.4%, 34.5%, 65.0%, and 42.9%, respectively. The hydrological effect of vegetation restoration was attributed to the annual precipitation, with a critical value of 1181 mm. Vegetation restoration increased annual depth of runoff when annual precipitation was less than the critical value, and increased that when annual precipitation was higher than the critical value. Therefore, the critical value might help to explain the difference in contribution rates of vegetation restoration to runoff in different watersheds and serve as one of the important reasons for the debate and divergence of forest restoration impacts on runoff.
    Simulating the post-fire net primary production restoration and its affecting factors by using MTCLIM and 3PGS model in Genhe forest region, Northeast China
    LIN Si-mei, HUANG Hua-guo
    2018, 29(11):  3712-3722.  doi:10.13287/j.1001-9332.201811.012
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    Fire is a major disturbance factor in Daxing’anling region, with important impacts on carbon balance of forest ecosystems. Fire severity and the distinction of microclimates induced by different topography are the primary factors driving the restoration of post-fire net primary productivity (NPP). In this study, we examined the influence of fire severity and topographic factors on the restoration of forest NPP in the Genhe forest region. The spatial and temporal restoration process of post-fire NPP were simulated by combining with MTCLIM and 3PGS model based on multiyear Landsat TM satellite (2008-2012) and climate (1980-2010) data. The results showed that the 3PGS-MTCLIM model could precisely estimate the spatial distribution of NPP at small scales, with a good correlation between simulated and observed values (R2=0.828). The percentage of declined NPP in the year following the fire ranged 43%-80%, and the average NPP recovery period for this region was about 10 years by comparing pre- and post-fire NPP. Fire severity had significant impacts on post-fire recovery. The stronger the fire intensity, the longer the recovery period was needed. The NPP recovered relatively slower after a period of fast-speed recovery. Among the three topographic factors, elevation was the strongest one affecting forest NPP restoration, followed by slope and aspect.
    Coupling analysis of landscape pattern and thermal fields after the afforestation in Beijing plain area
    JIN Jia-li, WANG Cheng, JIA Bao-quan
    2018, 29(11):  3723-3734.  doi:10.13287/j.1001-9332.201811.011
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    Under the positive human land use regulation (e.g. afforestation), understanding the impacts of landscape pattern changes on urban and rural thermal fields can provide reference data for future construction and optimization of urban afforestation projects and bring new insights for future landscape patterns and thermal fields environment related research. Under the context of “One Million Mu Plain Afforestation Project” in Beijing plain area, we applied remote sensing technology and geographic information system and used transfer-matrix method and coupling analysis to evaluate the impacts of plain afforestation on thermal field environment and to explore the correlation between changes in landscape patterns resulted from urban afforestation projects and urban land surface temperature. Our results showed that the percentage of landscape (PLAND) of forests and parks increased by 7.6% and 0.5% after the afforestation project in Beijing plain area, respectively. The heat island intensity decreased by 19.2% of entire plain area, and by 23.3% of suburban area, however, it increased by 23.5% of city area. Spanning urban to urban-rural interface, the PLAND of forests and parks, the mean patch size (MPS) of forests and wetlands were significantly negatively correlated with the changes in landscape surface temperature (LST). In city area, the area-weighted mean shape index (AWMSI) of forests was significantly positively correlated with the changes of LST. In suburban area, the MPS and AWMSI of forests showed significantly negative correlation with changes of LST, which was contrary to parks. After the implementation of afforestation project in Beijing plain area, the increased urban forests and urban parks had mitigated thermal fields to some extent. The scale of mitigation was limited and most areas with decreased heat island intensity were concentrated in newly planted sites in suburban area. The more connected the forests and wetlands, the better the relief of the thermal fields in the plain area. Impacted by the grain size, time, topography, human activities and other factors, the change in landscape patterns caused by the control of land use structure showed non-linear correlation with the change of LST.
    Temporal and spatial changes of urban impervious surface and its influence on urban ecolo-gical quality: A comparison between Shanghai and New York
    WANG Mei-ya, XU Han-qiu
    2018, 29(11):  3735-3746.  doi:10.13287/j.1001-9332.201811.018
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    The urban spatial expansion has led to the considerable substitution of natural vegetation-dominated land surfaces by impervious surfaces, especially in large cities, with great impacts on urban ecological quality. Two most heavily populated cities, Shanghai of China and New York of USA, were chosen as the study cases. Based on Landsat images obtained in 1989, 2002, 2015 in Shanghai and in 1991, 2001, 2015 in New York, normalized difference impervious surface index (NDISI) was used to extract impervious surface (IS) information. The remote sensing based ecolo-gical index (RSEI) was then applied to evaluate the changes of urban ecological quality caused by the increased impervious surface. Furthermore, landscape pattern indices were used to analyze the differences of spatial structure of impervious surface between Shanghai and New York and their influences on urban ecological quality. The results showed a significant difference in urban expansion rate and pattern between Shanghai and New York from the early 1990s to 2015. The IS expansion area in Shanghai was 17.4 times as much as that in New York. The annual IS increase rate of Shanghai was 62.2 times as much as that of New York. Shanghai had experienced an expansion from urban center to the surrounding countryside in a concentric ring pattern, whereas New York showed no much expansion but had IS increase mainly within the inner city through space filling pattern. These differences in IS change rate and spatial distribution pattern had resulted in the difference in urban ecological quality of the two cities. The mean RSEI in Shanghai dropped from 0.717 in 1989 to 0.453 in 2015, with a decrease of 36.8%. In contrast, the RSEI of New York had a decline of 6.9% from 0.552 in 1991 to 0.514 in 2015. The poor ecological condition urban area tended to have large IS patches that were well connected and aggregated.
    Non-monetary accounting on forest ecosystem services value: Case study of forests in Beijing-Tianjin-Hebei Region
    YANG Qing, LIU Geng-yuan
    2018, 29(11):  3747-3759.  doi:10.13287/j.1001-9332.201811.019
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    Forest ecosystem has significant functions and services. The accounting on forest ecosystem services valuation (ESV) is one of the key prerequisites to forest ESV pricing, ecosystems protection, and the implementation of that “Lucid waters and lush mountains are invaluable assets”. We first reviewed the advantages and limitations of current forest ESV counting methods, and then established the non-monetary accounting method on forest ESV based on emergy method. Further, we used forests in Beijing-Tianjin-Hebei area to test this accounting method. In addition, we modified the ESV accounting results based on normalized differential vegetation index (NDVI). Meanwhile, considering the uncertainty of data source, we conducted the uncertainty analysis on the accounting results. The results showed that the order of forest ESV in the different areas of Beijing-Tianjin-Hebei was Chengde > Beijing > Baoding > Zhangjiakou > Shijiazhuang > Qinhuangdao > Tianjin > Tangshan > Xingtai > Langfang > Hengshui >Cangzhou. The ESV order of different forest types was deciduous broad-leaved forest > evergreen coniferous forest > deciduous coniferous forest. In terms of different ecosystem services subtypes, the largest type of the ESV per unit area in Beijing-Tianjin-Hebei was climate regulation, accounting for around 60% of the total ESV per unit area, following by net primary productivity (NPP) increase and carbon sequestration, accounting for around 23%, and the proportion of other subtypes were small. The forest ESV in Hebei Province contributed most to local GDP, followed by Beijing and Tianjin. The non-monetary forest ESV accounting method established here could overcome the limitations of previous methods, including double counting, failure to establish ESV from donor side and the improper use or replacement of emergy transformities. We systematically assessed the forest ESV and could provide more effective optimization and decision-making basis on forest ecosystem services pricing, eco-compensation establishment and ecological civilization promotion.
    Multidimensional poverty measurement of poverty-stricken counties in China’s 14 conti-guous destitute areas considering ecological environment
    QI Wen-ping, WANG Yan-hui, ZHAO Wen-ji, LIAGN Chen-xia, CHENG Xu
    2018, 29(11):  3760-3772.  doi:10.13287/j.1001-9332.201811.013
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    As an important part of the national poverty alleviation strategy in China, monitoring of poverty-stricken counties is the precondition and guarantee for implementing national precise poverty reduction strategies. Taking 724 poverty-stricken counties in 14 contiguous destitute areas as the study area, we designed a county-level multidimensional poverty measurement index system that reflected the economic state (poverty appearance), social state (living guarantee ability) and ecological environment state (potential capacity of regional development) in fully consideration of the interdependent relationship between ecological vulnerability and economic poverty, and analyzed the poverty degree, poverty type, causes of poverty and the spatial distribution characteristics in multi-scale and multi-angle levels. The results showed that the distribution of poverty level of different counties represented a geometrically olive-shaped pattern that was larger in lower and moderate impoverished counties and smaller in low and high poverty-stricken counties. As far as the distribution of poverty-stricken counties was concerned, the distribution was spatially heterogeneous, presenting a typical stepped structure that was “west-high vs. east-low”, where different levels of dotted poverty kernels were scattered. The multidimensional characteristics of poverty-stricken counties represented a wholely strong spatial dependence; however, both High-High areas and Low-Low areas were distributed intensively while both High-Low areas and Low-High areas were distributed discretely. The characteristics of poverty distribution on both sides of the “Hu Line” were obviously different. The number of seven-factor comprehensive poverty-stricken counties was the most, while the location advantages, terrain conditions and poverty alleviation performance were the significant factors affecting the development of poverty reduction. Ecological poverty caused by deterioration of ecological environment and natural endowments is a regional characteristic in most poverty areas of China.
    Molecular weight fractionated characterization of dissolved organic matter and its correlation with water quality in the Bahe River Basin, Northwest China
    YUAN Bo, GUO Meng-jing, ZHENG Xing, ZHOU Xiao-de
    2018, 29(11):  3773-3782.  doi:10.13287/j.1001-9332.201811.015
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    Dissolved organic matter (DOM) is one of the core carriers of organic matter decomposition and nutrient regeneration in water. It is an important link of biogeochemical cycle of carbon and nitrogen, and the key content of water environmental science research. We used liquid chromatography-organic carbon detection-organic nitrogen detection (LC-OCD-OND) to investigate the molecular weight distribution of riverine DOM in the Bahe River of Xi’an City and further analyzed its correlation with water quality. The results showed that riverine DOM was composed of biopolymers, humic substances, humus degradation products, and low molecular-weight neutrals, with the ave-rage concentration of 0.15, 1.75, 0.48, 0.36 and 0.002 mg·L-1, respectively. The DOM along the river was in order of urban> town> river source. The humic substances with molecular weight of 1000-20000 Da accounted for 49.0% of total DOM which followed the order of town> waste water treatment plants (WWTPs) effluents>river estuary> river source. The biopolymers with the molecular weight of >20000Da accounted for 5.1% of total DOM, with the sequence of WWTPs effluents>river estuary>river source>town. The allochthonous (terrigenous) DOM produced by WWTPs effluents had the greatest contribution to the riverine DOM. The fractions of DOM with different molecular weights had significant correlations with water quality parameters. The results showed that the molecular weight fractions and abundance of DOM based on LC-OCD-OND could be used as a comprehensive index for water quality monitoring, to characterize the spatial heterogeneity of river water quality, and used for quantitative identification and source apportionment of pollutant components.
    Microbial biodiversity in rhizospheric soil of Torreya grandis ‘Merrillii’ relative to cultivation history
    YE Wen, LI Yong-chun, YU Wei-wu, YE Xiao-ming, QIAN Yu-ting, DAI Wen-sheng
    2018, 29(11):  3783-3792.  doi:10.13287/j.1001-9332.201811.034
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    To examine the effects of different cultivation history (5 a,10 a, and 15 a) on soil microbial communities, we used Illumina sequencing to investigate the diversity and structure of soil bacterial and fungal communities from Torreya grandis ‘Merrillii’ fields. The results showed that bacterial Shannon index, the richness estimators Chao1 and ACE were lower in soil in 15 year-old stand than those in other cultivation histories, while Simpson index showed no significant variation. Results from bacterial community NMDS showed that cultivation history played a vital role in driving the changes of soil bacteria communitiy structure. The bacterial communities in 5 and 10 year-old stand had the similar composition. The variations of bacterial richness and diversity as well as community structure (comprised basically of Proteobacteria, Actinobacteria, Acidobacteria, and Chloroflexi) were significantly correlated with soil organic matters, soil C/N, and total nitrogen. The fungi richness estimators of Chao1 and ACE were significantly decreased with increasing cultivation history. Shannon and Simpson indices were significantly higher in soil with 10 year-old stand than soils with other cultivation history. Fungal NMDS could be clustered in the same era. Fungal communities were comprosed of Ascomycota, Basidiomycota and Zygomycota. Changes in fungal richness/diversity and community structure were mainly controlled by the variation of soil organic matter. In conclusion, the predominant factors affecting soil microbial communities were the cultivation history, soil C/N, total nitrogen and organic matter, respectively.
    Relationship between soil bacterial community and environmental factors in the degraded alpine grassland of eastern Qilian Mountains, China
    LI Hai-yun, YAO Tuo, ZHANG Jian-gui, GAO Ya-min, MA Ya-chun, LU Xiao-wen, ZHANG Hui-rong, YANG Xiao-lei
    2018, 29(11):  3793-3801.  doi:10.13287/j.1001-9332.201811.037
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    To explore the distribution of bacterial community and its relationship with soil environmental factors in degraded alpine grasslands in the eastern Qilian Mountains, we analyzed the changes of bacterial community structure and diversity across lightly, moderately and severely degraded grasslands by using high-throughput sequencing technology. Redundancy analysis (RDA) was performed to analyze the relationship between soil bacterial communities and soil environmental factors by using CANOCO 4.5 software. The results showed that there were significant differences in soil physicochemical properties among different degraded alpine grasslands. There were 257125 effective sequences, 180826 high-quality sequences and 4790 OTUs. The Chao1 index was lightly degraded grassland > moderately degraded grassland > severely degraded grassland; Shannon index was lightly degraded grassland > severely degraded grassland > moderately degraded grassland. Phylogenetic analysis indicated that the soil bacterial groups of each plot belonged to 33 phyla, with Actinomycetes, Proteobacteria and Firmicutes being the dominant groups in the three grasslands. From analysis of the proportion of soil bacteria in different degraded grassland, we found that the Actinomycetes, Acidobacteria and Proteobacteria increased first and then decreased with the degree of degradation, and the Firmicutes showed an opposite trend. The results of RDA analysis showed that the dominant groups of bacteria were significantly correlated with invertase, cellulase, phosphatas, pH, electronic conductivity, available nitrogen and available potassium. It was concluded that there were significant differences in soil bacterial communities among different degraded alpine grasslands in the eastern Qilian Mountains, and the soil environmental factors were the important factors driving the distribution of soil bacterial communities.
    Comparison on fungal molecular ecological networks of agricultural soils with different latitudes in the black soil region of Northeast China
    HU Xiao-jing, LIU Jun-jie, WEI Dan, ZHU Ping, CUI Xi-an, ZHOU Bao-ku, CHEN Xue-li, JIN Jian, LIU Xiao-bing, WANG Guang-hua
    2018, 29(11):  3802-3810.  doi:10.13287/j.1001-9332.201811.033
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    To investigate the differences of fungal network structures and interaction among fungal species of in black soil region of Northeast China, Illumina MiSeq sequencing was used to reveal the fungal communities in the three long-term fertilization experimental fields. Fungal molecular ecological networks were constructed based on random matrix theory (RMT). The results demonstrated that Ascomycota, Basidiomycota and Zygomycota were the dominant phyla and Hypocreales, Pleosporales and Sordariales were the dominant order, but the relative abundance of some dominant taxa significantly varied in different locations. Fungal molecular ecological network structures in three locations showed significant difference, with more complex fungal network being observed in north location with more competitive relations among species. The fungal network in south location was more easily disturbed by environmental perturbations with less stability. Only seven shared nodes were detected among three fungal molecular ecological networks. There were large differences in connectivity of shared nodes within individual fungal network. The subnetwork of Hypocreales was gradual complex from south to north location while subnetwork of Pleosporales presented reversed trend. The key species of south, middle and north locations were Chaetomiaceae, Pleosporales and Penicillium coralligerum, respectively. Soil pH and total N content were the main soil properties simultaneously influencing three fungal networks.
    Colonization with arbuscular mycorrhizal fungus Funneliformis mosseae enhanced the responses of tomato plants to mechanical wounding
    SONG Yuan-yuan, XIA Ming, LIN Yi-bin, LIN Xian-hui, DING Chao-hui, WANG Jie, HU Lin, ZENG Ren-sen
    2018, 29(11):  3811-3818.  doi:10.13287/j.1001-9332.201811.035
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    Insect herbivore feeding causes mechanical damage to plants, which can activate plant defense responses. Whether symbiosis with beneficial microorganisms can enhance the responses of plants to mechanical damage is of importance for plant anti-herbivore resistance. In this study, defense responses of tomato (Lycopersicon esculentum) plants to mechanical wounding was investigated after the tomato roots being infected by arbuscular mycorrhizal fungus (AMF) Funneliformis mosseae. The results showed that in response to leaf mechanical wounding, the activities of phenylalanine ammonia-lyase (PAL), superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO) and catalase (CAT) in the leaves of tomato pre-inoculated with AMF (FD), as well as transcript levels of genes encoding phenylalanine ammonia lyase (PAL) and β-1,3-glucanase (PR2) in the leaves and roots were significantly higher in relative to sole mechanical wounding (D), sole mycorrhizal inoculation (F), and control without mechanical wounding and mycorrhizal inoculation (CK). Although the activities of protective enzyme and transcript levels of the two defense-related genes were induced in the plants of sole mechanical wounding (D) and sole mycorrhizal inoculation (F), the induction was faster and stronger in the plants with leaf mechanical wounding and mycorrhizal pre-inoculation (FD). Our findings indicated that arbuscular mycorrhizal colonization could prime quicker and stronger defense responses of tomato plants to mechanical damage.
    Root promoting effect and its regulation mechanisms of endophytic Bacillus amyloliquefaciens YTB1407 in sweet potato
    WANG Cui-juan, WANG Ying-zi, CHU Zhao-hui, WANG Pei-song, LUAN Bing-hui
    2018, 29(11):  3819-3828.  doi:10.13287/j.1001-9332.201811.040
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    We isolated the endophytic Bacillus amyloliquefaciens YTB1407 from the root of Panax quinquefolium, which has both biological control and growth promoting effects. To investigate its potential applications, a pot experiment of sweet potato was tested to assess the capacity of endophytic colonization of YTB1407 and the selection of its optimum concentration by investigating the performance of root characteristics on three time points in the whole early growth phase after irrigating with different concentrations of bacterial suspensions with treatment of sterile water as control. The activities of endogenous hormone IAA, ZR, t-ZR and IAA oxidase (IAAO, PPO, POD) were analyzed. The results showed that YTB1407 promoted the specific colonization of root system, the elongation of adventitious root and branch roots, and root activity in the early growth stage of sweet potato. At later growth stage, it formed greater fresh mass of absorption root and lower aboveground/root system mass ratio. YTB1407 suspensions with OD600 of 0.50 (T0.50) had more significant effect, which induced the highest fresh tuber mass and the largest effective tuber numbers of per plant at top cover stage. YTB1407 promoted the differentiation of adventitious roots into tubers at initial point of tuberization by increasing IAA content and the ratio of (t-ZR+ZR)/IAA, decreasing IAAO activity and enhancing PPO activity. Moreover, it promoted the differentiated roots into tubers at tuberization stage by keeping the higher content of IAA, lower ratio of (t-ZR+ZR)/IAA, and decreasing IAAO and PPO activities.
    Effects of long-term different fertilization regimes on the abundance and community structure of ammonia oxidizers in paddy soils
    YANG Ya-dong, SONG Run-ke, ZHAO Jie, WANG Pei-xin, XU Xiao-ling, ZENG Zhao-hai
    2018, 29(11):  3829-3837.  doi:10.13287/j.1001-9332.201811.031
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    Ammonia oxidation, driven by the ammonia oxidizers, is the rate-limiting step of nitrification and plays a key role in soil nitrogen cycling. In this study, real-time PCR and Illumina MiSeq sequencing approaches were used to investigate the effects of long-term different fertilization regimes on the abundance and community structure of ammonia oxidizers, targeting the amoA genes, in a 30-year located experimental paddy soil in Ningxiang County, Hunan Province. Four treatments were compared, including control without fertilizer (CK), fertilizers NPK (CF), 70% NPK plus 30% manure (CFM1), and 40% NPK plus 60% manure (CFM2). The results showed that the abundance of amoA genes in AOA and AOB was in the range of 3.09×107-8.37×107 and 1.04×107-7.03×107 copies·g-1 dry soil, respectively. Fertilization significantly increased the AOA and AOB abundances. However, no significant difference was observed in AOB abundance between CFM2 and CK. Manure fertilization rate greatly affected the α diversity index of AOB rather than AOA. The Shannon index of AOA and ACE and Chao1 indexes of AOB observed in CFM1 were significantly higher than that in CK, respectively. Thaumarchaeota and Crenarchaeota were the predominant AOA phyla and Nitrosospira, environmental_samples_norank, Bacteria_unclassified and Nitrosomonadales_unclassified were the main AOB genus groups which accounted for 83.4% and 97.8% of the total AOA and AOB amoA gene reads, respectively. Venn diagram indicated that manure fertilization rate had a stronger effect on the OTU number of AOB amoA gene than that of AOA in different treatments, but it slightly altered the proportion of shared AOA and AOB amoA gene reads. Furthermore, there were pronounced differences in the community structure of AOB among different treatments than that of AOA. These results suggested that manure fertilization rate significantly affected the abundance, diversity and community structure of AOA and AOB. The Shannon index of AOA and the abundance and ACE and Chao1 indexes of AOB in CFM1 were significantly higher than that in the rest treatments, respectively. Our results provided basis for further exploring the response mechanism of ammonia oxidizers to different fertilization strategies and the roles of ammonia oxidizers in nitrogen transformation in agricultural systems.
    Behavioral responses of Holotrichia oblita larvae to root exudates from three host plants
    MA Yan-hua, LI Xue, CAO Ya-zhong, YIN Jiao, ZHANG Shuai, LI Ke-bin
    2018, 29(11):  3838-3846.  doi:10.13287/j.1001-9332.201811.032
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    The larva of Holotrichia oblita is a serious soil pest that feed with plant roots in north China. To explore the effects of host root exudates on the larva could provide theoretical basis for the development of green prevention and control methods. In order to elucidate the behavioral responses of Holotrichia oblita larva to the roots of peanut, soybean and maize, an experiment was conducted using the Y-olfactometers with the air as control. The constituents of the root exudates from the three host plants were identified by the gas chromatography-mass spectrometer (GC-MS). The olfactory responses of H. oblita larvae to the main components were tested. The results showed that H. oblita larvae had a significant behavioral preference toward the roots of peanut, soybean and maize than the control. The GC-MS analysis showed that the main components of volatile compounds in the three plants had more than twenty compounds, with only three shared ones, which was glycerol, dodecanol, ethyl benzene. The results of the Y-olfactometers showed that at low concentrations (40 to 80 μg·mL-1), the number of insects attracted by compound 2-butenoic acid, methylsuccinic acid, myristic acid, acetic acid and phthalate was significantly higher than that of control. At the concentrations of 100 μg·mL-1, 200 μg·mL-1, compound tetradecane and hexadecane were more attractive to the larvae than the control. The compounds p-xylene, o-xylene, and palmitic acid glycerol were found to significantly induce the larva at the concentration of 300 μg·mL-1 and 500 μg·mL-1. In summary, the main components of plant root exudates had a significant luring effect on H. oblita larvae.
    Contamination status and speciation for the sediment nutrients in Panjiakou-Daheiting Reservoir
    ZHU Xiang, ZHANG Min, QU Xiao-dong, PENG Wen-qi, DUAN Long-fei
    2018, 29(11):  3847-3856.  doi:10.13287/j.1001-9332.201811.036
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    As the source of the “Luanhe-Tianjin Diversion Project”, water environment status in the Panjiakou-Daheiting Reservoir directly affects drinking water safety in the water receiving area, and therefore plays important role in the development of the Beijing-Tianjin-Hebei region. Field survey was carried out in July 2016 to examine the pollution status of the sediment nitrogen, phospho-rus and organic matter. The results showed that total nitrogen (TN) content of surface sediments in Panjiakou-Daheiting Reservoir ranged from 1175.41 to 2415.67 mg·kg-1 with a mean of 1648.71 mg·kg-1. Total phosphorus (TP) ranged from 1773.25 to 3471.70 mg·kg-1 with a mean of 2790.89 mg·kg-1. Organic matter (OM) ranged from 6.0% to 25.3% with a mean of 12.5%. Daheiting Reservoir which located downstream of Panjiakou Reservoir had higher value in TP content, while had similar TN content. Results from speciation analysis showed that sediment nitrogen was mainly composed of organic nitrogen and phosphorus mainly consisted of calcium-phosphorus which belonged to inorganic phosphorus. The value of C/N ratio indicated that OM mainly originated from exogenous substances. The pollution assessment results demonstrated that the sediment Panjiakou-Daheiting Reservoir was in a severe pollution status, and might have potential effect to the water environment improvement through the nutrient exchange at the sediment-water interface.
    Community structure of Chironomid larvae and their indicative significance for water quality in streams of Xianju National Park, China
    ZHOU Dong, ZHANG Wei, ZHU Li-ming, QI Xin, WANG Li-qing, ZHANG Rui-lei
    2018, 29(11):  3857-3866.  doi:10.13287/j.1001-9332.201811.039
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    In August 2016, 59 sites of 13 streams in Xianju National Park were surveyed to explore the community structure of Chironomid larvae and their indicative significance for water quality. Water quality of the streams was evaluated based on Hilsenhoff biological index. The results showed that there were 37 clean sites, 19 mild polluted sites and three moderated polluted sites in Xianju National Park. We identified a total of 86 species of Chironomid larvae, belonging to 51 genera and four subfamilies, including 37 species in 19 genera of Chironominae; 35 species in 21 genera of Orthcladiinae; 12 species in 9 genera of Tanypodinae, two species in two genera of Diamesinae. Chironominae accounted for 40.3% of Chironomid larval density. Tanypodinae and Orthcladiinae were almost similar in Chironomid larval density, respectively accounted for 30% and 29.4% of the total. Diamesinae accounted for 0.3% of total Chironomid larval density. Conchapelopia sp. was the overwhelming dominant species and Polypedilum flavum, Rheocricotopus sp., Cricotupus vierriensis, Rheotanytarsus muscicola and Corynoneura carriana were the dominant species of Chironomid larvae. The result of indicator species analysis showed that Cricotupus vierriensis, Nanocladius dichromus, Tanytarsus formosanus, Tanytarsus mendex were indicator species of moderated pollution water, and that Rheocricotopus sp. was the indicator species of clean state water.
    Niche and interspecific associations of major nekton in northern coastal waters in Yuhuan, Taizhou, China
    ZHANG Miao-miao, WANG Yong-xue, TIAN Kuo, SUN Peng, QIU Jin-jin, NIE Zhen-lin, TIAN Jia-qi, SHUI Bo-nian
    2018, 29(11):  3867-3875.  doi:10.13287/j.1001-9332.201811.038
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    Base on the data from fishery resources survey carried out in northern coastal waters in Yuhuan in November 2016 (autumn) and April 2017 (spring), we examined the niche and interspecific associations of major nekton by the methods of index of relative importance (IRI), niche breadth, niche overlap, the variance ratio (VR), X2-test, association coefficient (AC), Jaccard index (JI), point correlation coefficient (Φ) and Spearman rank correlation index. The ecological niche breadth of Portunus trituberculatus was the largest, with the absolute competitive advantage. The niche breadths of the important economic species Coilia nasus and Octopus variabilis were the smallest, which was at a weak competitive position. The niche overlap of the major nekton species in this area was uneven, possibly caused by the diversity of local environments. Overall interspecific associations indicated that insignificant negative correlation existed among major nekton species. All results of the X2-test, association coefficient (AC) and Jaccard index (JI) were consistent with that of overall interspecific associations, which indicated the independent trend among species. The correlation coefficients (Φ) and the Spearman rank correlation index revealed that the positive coupling pairs were more than the negative ones. It was due to the positive association among the other 14 species, caused by the spatial exclusion from large captured individuals of Muraenesox cinereus. According to the Spearman rank correlation and cluster analysis, 15 species could be divided into five ecological groups. The species within one ecological group had similar ecologically adaptable requirements of habitat, while the species from different ecological groups had different living habits and different ecological requirements.
    Jasmonates: From induced plant anti-herbivore defensive reaction to growth-defense tradeoffs
    JIAO Long, CAI Xiao-ming, BIAN Lei, LUO Zong-xiu, LI Zhao-qun, XIN Zhao-jun, CHEN Zong-mao
    2018, 29(11):  3876-3890.  doi:10.13287/j.1001-9332.201811.030
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    Jasmonates (JAs), which can exogenously induce anti-herbivore defensive reaction in plants, are important in the field of plant physiology and plant protection. We summarized the ecological effects of exogenous JAs applied on 40 species of plants on various herbivores (such as Lepidoptera and Hemipteran) and their natural enemies in the past 20 years. We systematically genera-lized the research status about the direct and indirect antiherbivore defense induced by JAs, induced systemic defense, induction methods, induced performance in field, and the application status of JAs. Moreover, combining with the latest literatures, we reviewed the effects of JAs on plant growth-defense trade-offs from the cross-talk between phytohormone signaling pathways, and the regulation of nodes in the JA signaling pathway. Finally, we proposed the future directions and key aspects of the research on the plant anti-herbivore defense induced by exogenous JAs, which would promote the development of the related research and the application of JAs in field.