Effects of exogenous salicylic acid and brassinolide on photosynthesis of cucumber seedlings under low temperature stress.

doi:10.13287/j.1001-9332.201609.020

Abstract

Abstract: An experiment was conducted to investigate the mechanism of the regulation of salicylic acid (SA) and 2,4-epibrassionolide (EBR) on photosynthesis in cucumber (Cucumis sativa) seedlings under low temperature stress. Taking cucumber cultivar ‘Youbo1-5’ as material, the seedlings were pre-treated with 1 mmol·L^-1 SA or 0.1 μmol·L^-1EBR (sprayed once a day), and then were exposed to chilling temperature (10 ℃/5 ℃, PFD 80 μmol·m^-2·s^-1) after being pre-treated 2 days. The results showed that the growth and net photosynthetic rate (P_n) of cucumber seedlings were decreased under low temperature stress. However, the P_n, maximal photochemical efficiency of PSⅡ (F_v/F_m), actual photochemical efficiency of PSⅡ (Φ_PS_Ⅱ) and photochemical quenching coefficient (q_P) were significantly improved in SA- and EBR-pretreated seedlings, and the increase range of non-photochemical quenching coefficient (NPQ) was decreased. Moreover, the activities of ribulose 1,5-biphosphate carboxylase/oxygenase (Rubisco), sedoheptulose-1,7-bisphosphatase (SBPase), fructose-1,6-bisphosphate aldolase (FBA) and transketolase (TK) were signi-ficantly increased. These findings suggested that SA and EBR improved photosynthesis of cucumber seedlings by promoting the activities of key enzymes and increased low temperature tolerance.

XU Xiao-yun, YU Ji-hua, XIE Jian-ming, HU Lin-li, LI Jie. Effects of exogenous salicylic acid and brassinolide on photosynthesis of cucumber seedlings under low temperature stress.[J]. Chinese Journal of Applied Ecology, 2016, 27(9): 3009-3015.

References

[1] Saltveit ME. Chilling injury is reduced in cucumber and rice seedlings and in tomato pericarp discs by heat-shocks applied after chilling. Postharvest Biology and Technology, 2001, 21: 169-177
[2] Sun J-L (孙军利), Zhao B-L (赵宝龙), Yu S-L (郁松林). Study of exogenous salicylic acid (SA) on the heat tolerance in grape seedlings under high temperature stress. Journal of Soil and Water Conservation (水土保持学报), 2014, 28(3): 290-294 (in Chinese)
[3] Sun J-L (孙军利), Zhao B-L (赵宝龙), Yu S-L (郁松林). Study of exogenous salicylic acid on antioxidant enzymes activities and antioxidants contents in grape seedlings under high temperature stress. Plant Physiology Journal (植物生理学报), 2014, 50(7): 1014-1018 (in Chinese)
[4] Zhou M-M (周萌萌), Gu J-T (谷建田), Zhang X-C (张喜春), et al. Effects of 5-sulfosalicylic acid on physiological-biochemical characteristics of tomato under low temperature stress. Chinese Agricultural Science Bulletin (中国农学通报), 2015, 31(31): 68-73 (in Chinese)
[5] Huang Y-M (黄玉梅), Zhang Y-X (张杨雪), Liu Q-L (刘庆林), et al. Effects of salicylic acid on seed germination and seedling physiological characteristics of Zinnia elegans under salt stress. Acta Prataculturae Sinica (草业学报), 2015, 24(7): 97-105 (in Chinese)
[6] Zhang Y-P (张永平), Fan H-W (范红伟), Yang S-J (杨少军), et al. Alleviating effects of exogenous salicylic acid on growth, photosynthesis and reactive oxygen metabolism in melon seedlings under cadmium stress. Plant Physiology Journal (植物生理学报), 2014, 50(10): 1555-1562 (in Chinese)
[7] Wang L-J (王丽君), Zhang G-B (张国斌), Zhou Y-T (周亚婷), et al. Effects of exogenous salicylic acid on antioxidant capacity in cauliflower leaves under drought stress. Chinese Agricultural Science Bulletin (中国农学通报), 2014, 30(25): 91-96 (in Chinese)
[8] Li L-L (李琳琳), Li T-L (李天来), Jiang G-B (姜国斌), et al. Synergistion mechanism of exogenous Ca²⁺ to SA-induced resistance to Botrytis cinerea in tomato. Chinese Journal of Applied Ecology (应用生态学报), 2015, 26(11): 3497-3502 (in Chinese)
[9] Xue R-F (薛仁风), Wu J (武晶), Zhu Z-D (朱振东), et al. Salicylic acid increase resistance to Fusa-rium oxysporum f. sp. phaseoli in common beans (Phaseolus vulgaris L.). Journal of Plant Genetic Resources (植物遗传资源学报), 2014, 15(5): 1138-1143 (in Chinese)
[10] Wang Q-Y (王庆燕), Guan D-H (管大海), Pan H-B (潘海波), et al. Effects of brassinolide on leaf photosynthetic function and yield in spring maize filling stage. Acta Agronomica Sinica (作物学报), 2015, 41(10): 1557-1563 (in Chinese)
[11] Ma J-H (马金虎), Xing G-F (邢国芳), Yang X-H (杨小环), et al. Effects of exogenous EBR and NO signal on antioxidant system and low response gene expression under cold stress on maize embryo. Chinese Journal of Applied Ecology (应用生态学报), 2015, 26(5): 1411-1418 (in Chinese)
[12] Guo F (郭芳), Wang Y-J (王亚军), Xie Z-K (谢忠奎), et al. The effects of inducers on growth, disease resistance and related enzymes activities of lily cut flo-wer. Acta Horticulturae Sinica (园艺学报), 2010, 37(5): 763-768 (in Chinese)
[13] Shu H-M (束红梅), Guo S-Q (郭书巧), Gong Y-Y (巩元勇), et al. Effects of brassionlide on leaf physiological characteristics and differential gene expression profiles of Nacl-stressed cotton. Chinese Journal of Applied Ecology (应用生态学报), 2016, 27(1): 150-156 (in Chinese)
[14] Fariduddin Q, Yusuf M, Chalkoo S, et al. 28-homobrassinolide improves growth and photosynthesis in Cu-cumis sativus L. through an enhanced antioxidant system in the presence of chilling stress. Photosynthetica, 2011, 49: 55-64
[15] Li J (李杰), Yang P (杨萍), Xie J-M (颉建明), et al. Effects of 2, 4-epibrassinolide on growth and antioxidant enzymes system in pepper roots under chil-ling stress. Journal of Nuclear Agricultural Sciences (核农学报), 2015, 29(5): 1001-1008 (in Chinese)
[16] Sayyari M. Improving chilling resistance of cucumber seedlings by salicylic acid. American-Eurasian Journal of Agricultural & Environmental Sciences, 2012, 12: 204-209
[17] Fariduddin Q, Yusuf M, Chalkoo S, et al. 28-homobrassinolide improves growth and photosynthesis in Cu-cumis sativus L. through an enhanced antioxidant system in the presence of chilling stress. Photosynthetica, 2011, 49: 55-64
[18] Rao IM, Terry N. Leaf phosphate status, photosynthesis, and carbon partitioning in sugar beet. I. Changes in growth, gas exchange, and Calvin cycle enzymes. Plant Physiology, 1989, 90: 814-819
[19] Tao H-Z (陶宏征), Zhao C-L (赵昶灵), Li W-Q (李唯奇). Photosynthetic response to low temperature in plant. Chinese Journal of Biochemistry and Molecular Bio-logy (中国生物化学与分子生物学报), 2012, 28(6): 501-508 (in Chinese)
[20] Allen DJ, Ort DR. Impacts of chilling temperatures on photosynthesis in warm-climate plants. Trends in Plant Science, 2001, 6: 36-42
[21] Hu WH, Wu Y, Zeng JZ, et al. Chill-induced inhibition of photosynthesis was alleviated by 24-epibrassino-lide pretreatment in cucumber during chilling and subsequent recovery. Photosynthetica, 2010, 48: 537-544
[22] Liu W (刘伟), Ai X-Z (艾希珍), Liang W-J (梁文娟), et al. Effects of salicylic acid on the leaf photosynthesis and antioxidant enzyme activities of cucumber seedlings under low temperature and light intensity. Chinese Journal of Applied Ecology (应用生态学报), 2009, 20(2): 441-445 (in Chinese)
[23] Hartley IP, Armstrong AF, Murthyw R, et al. The dependence of respiration on photosynthetic substrate supply and temperature: Integrating leaf, soil and ecosystem measurements. Global Change Biology, 2006, 12: 1954-1968
[24] Liang F (梁芳), Zheng C-S (郑成淑), Sun X-Z (孙宪芝), et al. Effects of low temperature and weak light stress and its recovery on the photosynthesis and chlorophyll fluorescence parameters of cut flower chrys-anthemum. Chinese Journal of Applied Ecology (应用生态学报), 2010, 21(1): 29-35 (in Chinese)
[25] Khokon MD, Okuma E, Hossain MA, et al. Involvement of extracellular oxidative burst in salicylic acid-induced stomatal closure in Arabidopsis. Plant, Cell & Environment, 2011, 34: 434-443
[26] Poór P, Tari I. Regulation of stomatal movement and photosynthetic activity in guard cells of tomato abaxial epidermal peels by salicylic acid. Functional Plant Bio-logy, 2012, 39: 1028-1037
[27] Dong F-C (董发才), Wang P-T (王朋涛), Zhang L (张霖), et al. The role of hydrogen peroxide in salicylic acid-induced stomatal closure in Vicia fabal guard cells. Plant Physiology Journal (植物生理学报), 2001, 27(4): 296-302, 361 (in Chinese)
[28] Liu X (刘新), Meng F-X (孟繁霞), Zhang S-Q (张蜀秋), et al. Ca²⁺ is involved in the signal transduction during stomatal movement in Vicia fabal L. induced by salicylic acid. Journal of Plant Physiology and Molecular Biology (植物生理与分子生物学学报), 2003, 29(1): 59-64 (in Chinese)
[29] Wu X (吴秀), Lu X-M (陆晓民). Effects of brassionlide on the antioxidant system and photosynthesis of cucumber seedlings under suboptimal temperature, light and salt environment. Chinese Journal of Applied Ecology (应用生态学报), 2015, 26(9): 2751-2757 (in Chinese)
[30] Zhang F (张帆), Yu J-H (郁继华), Xie J-M (颉建明), et al. Effects of exogenous ALA and CTS on photosynthesis and antioxidant enzymes of pepper seedlings under suboptimal temperature and light intensity. Arid Land Geography (干旱区地理), 2014, 37(1): 195-202 (in Chinese)
[31] Maxwell K, Johnson GN. Chlorophyll fluorescence: A practical guide. Journal of Experimental Botany, 2000, 51: 659-668
[32] Cao G (曹刚), Zhang G-B (张国斌), Yu J-H (郁继华), et al. Effects of different LED light qualities on cucumber seedling growth and chlorophyll fluorescence parameters. Scientia Agricultura Sinica (中国农业科学), 2013, 46(6): 1297-1304 (in Chinese)
[33] Huner NPA, Öquist G, Sarhan F. Energy balance and acclimation to light and cold. Trends in Plant Science, 1998, 3: 224-230
[34] Gutteridge S, Parry MAJ, Burton S, et al. A nocturnal inhibitor of carboxylation in leaves. Nature, 1986, 324: 274-276
[35] Stitt M, Lunn J, Usadel B. Arabidopsis and primary photosynthetic metabolism: More than the icing on the cake. The Plant Journal, 2010, 61: 1067-1091
[36] Harrison EP, Olcer H, Lloyd JC, et al. Small decreases in SBPase cause a linear decline in the apparent RuBP regeneration rate, but do not affect Rubisco carboxylation capacity. Journal of Experimental Botany, 2001, 52: 1779-1784
[37] Raines CA. The Calvin cycle revisited. Photosynthesis Research, 2003, 75: 1-10
[38] Bi G-H (毕焕改), Dong X-B (董绪兵), Wang M-L (王美玲), et al. Foliar spray calcium and salicylic acid improve the activities and gene expression of photosynthetic enzymes in cucumber seedlings under low light intensity and suboptimal temperature. Acta Horticulturae Sinica (园艺学报), 2015, 42(1): 56-64 (in Chinese)