Effects of nitrogen additions on soil hydrolase and oxidase activities in Pinus elliottii plantations.

doi:10.13287/j.1001-9332.201611.016

Abstract

Abstract: We evaluated responses of hydrolase and oxidase activities in a subtropical Pinus elliottii plantation through a nitrogen (N) addition field experiment (dosage level: 0, 40, 120 kg N·hm^-2·a^-1). The results showed that N additions significantly decreased the carbon, nitrogen and phosphorus related hydrolase and oxidase activities. The activities of β-1,4-glucosidase (BG), cellobiohydrolase (CBH), β-1,4-N-acetylglucosaminidase (NAG) and peroxidase (PER) activities were decreased by 16.5%-51.1% due to N additions, and the decrease was more remarkable in the higher N addition treatment. The activities of α-1,4-glucosidase (aG), β-1,4-xylosidase (BX), acid phosphatase (AP) and phenol oxidase (PPO) were decreased by 14.5%-38.6% by N additions, however, there was no significant difference among the different N addition treatments. Soil enzyme activities varied obviously in different seasons. The activities of BG, NAG, BX, CBH, AP and PPO were in the order of March > June > October, and aG and PER activities were in the order of October > March > June. Most of the soil hydrolase and oxidase activities were positively correlated with soil pH, but negatively with NO₃^--N content. It indicated that N additions inhibited soil hydrolase and oxidase activities by reducing soil pH and increasing soil nitrification. N additions inhibited the soil organic matter mineralization and turnover in the subtropical area, and the effects were obvious with the increasing dosage of N additions.

Key words: Pinus elliottii, red soil, nitrogen addition, hydrolase activity, oxidase activity

ZHANG Chuang, ZOU Hong-tao, ZHANG Xin-yu, KOU Liang, YANG Yang, SUN Xiao-min, LI Sheng-gong, WANG Hui-min. Effects of nitrogen additions on soil hydrolase and oxidase activities in Pinus elliottii plantations.[J]. Chinese Journal of Applied Ecology, 2016, 27(11): 3427-3434.

References

[1] Galloway JN, Dentener FJ, Capone DG, et al. Nitrogen cycles: Past, present, and future. Biogeochemistry, 2004, 70: 153-226
[2] Liu XJ, Duan L, Mo JM, et al. Nitrogen deposition and its ecological impact in China: An overview. Environmental Pollution, 2011, 159: 2253-2254
[3] Jia YL, Yu GR, He NP, et al. Spatial and decadal variations in inorganic nitrogen wet deposition in China induced by human activity. Scientific Peports, 2014, 4: 3763
[4] Huang J, Mo JM, Zhang W, et al. Research on acidification in forest soil driven by atmospheric nitrogen deposition. Acta Ecologica Sinica, 2014, 34: 304-306
[5] Gao WL, Yang H, Kou L, et al. Effects of nitrogen deposition and fertilization on N transformations in forest soils: A review. Journal of Soil and Sediments, 2015, 15: 863-875
[6] Treseder KK. Nitrogen additions and microbial biomass: A meta-analysis of ecosystem studies. Ecology Letters, 2008, 11: 1114-1118
[7] Sinsabaugh RL. Phenol oxidase, peroxidase and organic matter dynamics of soil. Soil Biology and Biochemistry, 2010, 24: 391-401
[8] Burns RG, DeForest JL, Marxsen J, et al. Soil enzymes in a changing environment: Current knowledge and future directions. Soil Biology and Biochemistry, 2013, 58: 216-227
[9] Kang H, Lee D. Inhibition of extracellular enzyme acti-vities in a forest soil by additions of inorganic nitrogen. Communications in Soil Science and Plant Analysis, 2005, 36: 2129-2133
[10] Dong WY, Zhang XY, Liu XY, et al. Responses of soil microbial communities and enzyme activities to nitrogen and phosphorus additions in Chinese fir plantations of subtropical China. Biogeosciences, 2015, 12: 5540-5544
[11] Sinsabaugh RL, Carreiro MM, Repert DA. Allocation of extracellular enzymatic activity in relation to litter composition, N deposition, and mass loss. Biogeochemistry, 2002, 60: 6-22
[12] Song YY, Song CC, Li YC, et al. Short-term effect of nitrogen addition on litter and soil properties in Calamagrostis angustifolia freshwater marshes of Northeast China. Wetlands, 2013, 33: 508-511
[13] Tu L-H (涂丽华), Hu H-L (胡红玲), Hu T-X (胡庭兴), et al. Effects of simulated nitrogen deposition on soil enzyme activities in a Betula luminifera plantation in Rainy Area of West China. Chinese Journal of Applied Ecology (应用生态学报), 2012, 23(8): 2129-2133 (in Chinese)
[14] Guo P, Wang CY, Jia Y, et al. Response of soil microbial biomass and enzymatic activities to fertilizations of mixed inorganic and organic nitrogen at a subtropical forest in East China. Plant and Soil, 2011, 338: 357-361
[15] Liu X (刘星), Wang J-S (汪金松), Zhao X-H (赵秀海). Effects of simulated nitrogen deposition on the soil enzyme activities in a Pinus tabulaeformis forest at the Taiyue Mountain. Acta Eclogica Sinica (生态学报), 2015, 35(14): 4620-4622 (in Chinese)
[16] Fang X (方晰), Tian D-L (田大伦), Xiang W-H (项文化), et al. On carbon accumulation, distribution of different densities in slash pine plantation. Journal of Zhejiang Forestry College (浙江林学院学报), 2003, 20(4): 374-375 (in Chinese)
[17] Kou L, Chen WW, Zhang XY, et al. Differential responses of needle and branch order-based root decay to nitrogen addition: Dominant effects of acid-unhydroly-zable residue and microbial enzymes. Plant and Soil, 2015, 394: 318-319
[18] Zhu JX, He NP, Wang QF, et al. The composition, spatial patterns, and influencing factors of atmospheric wet nitrogen deposition in Chinese terrestrial ecosystems. Science of the Total Environment, 2015, 511: 779-780
[19] Saiya-Cork KR, Sinsabaugh RL, Zak DR. The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biology and Biochemistry, 2002, 34: 1309-1314
[20] Stone MM, DeForest JL, Plante AF. Changes in extracellular enzyme activity and microbial community structure with soil depth at the Luquillo Critical Zone Obser-vatory. Soil Biology and Biochemistry, 2014, 75: 240-241
[21] Kim H, Kang H. The impacts of excessive nitrogen additions on enzyme activities and nutrient leaching in two contrasting forest soils. Journal of Microbiology, 2011, 49: 369-373
[22] Qian C, Cai ZC. Leaching of nitrogen from subtropical soils as affected by nitrification potential and base cations. Plant and Soil, 2007, 300: 199-204
[23] Boot CM, Hall EK, Denef K, et al. Long-term reactive nitrogen loading alters soil carbon and microbial community properties in a subalpine forest ecosystem. Soil Bio-logy and Biochemistry, 2015, 92: 215-218
[24] Janssens IA, Dieleman W, Luyssaert S, et al. Reduction of forest soil respiration in response to nitrogen deposition. Nature Geoscience, 2010, 3: 315-320
[25] Kou L, Guo DL, Yang H, et al. Growth, morphological traits and mycorrhizal colonization of fine roots respond differently to nitrogen addition in a slash pine plantation in subtropical China. Plant and Soil, 2015, 391: 210-216
[26] Kivlin SN, Treseder KK. Soil extracellular enzyme acti-vities correspond with abiotic factors more than fungal community composition. Biogeochemistry, 2014, 117: 24-34
[27] Fan J-J (樊金娟), Li D-D (李丹丹), Zhang X-Y (张心昱), et al. Temperature sensitivity of soil organic carbon mineralization and β-glucosidase enzyme kinetics in the northern temperate forests at different altitudes, China. Chinese Journal of Applied Ecology (应用生态学报), 2016, 27(1): 17-23 (in Chinese)
[28] Cao H (曹慧), Sun H (孙辉), Yang H (杨浩), et al. A review soil enzyme activities and its indication for soil quality. Chinese Journal ofApplied and Environmental Biology (应用与环境生物学报), 2003, 9(1): 105-108 (in Chinese)
[29] Kuzyakov Y, Blagodatskaya E. Microbial hotspots and hot moments in soil: Concept and review. Soil Biology and Biochemistry, 2015, 83: 184-195
[30] Razavi BS, Zarebanadkouki M, Blagodatskaya E, et al. Rhizosphere shape of lentil and maize: Spatial distribution of enzyme activities. Soil Biology and Biochemistry, 2016, 96: 229-236