Effects of calcium and ABA on photosynthesis and related enzymes activities in cucumber seedlings under drought stress

doi:10.13287/j.1001-9332.201612.025

Abstract

Abstract: To investigate the effect of calcium and ABA on photosynthesis and the activities of antioxidant enzymes in cucumber seedlings under drought stress, the cucumber was used as the expe-riment materials, normal nutrient solution culture was considered as the control, and PEG-6000 application in the nutrient solution simulated the drought stress. There were five different treatments which were spraying water, ABA, CaCl₂+ABA, LaCl₃(calcium channel inhibitor)+ABA and EGTA (calcium ion chelating agent)+ABA under drought stress. The results showed that drought stress inhibited the growth of cucumber seedlings, and reduced the activities of antioxidant enzymes, nitrate reductase, net photosynthetic rate and fluorescence parameters of the cucumber seedlings leaves. The application of ABA reduced the inhibition of activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), photosynthesis (P_n, g_s) and the fluorescence parameters (F_v′/F_m′, q_P and ETR), and decreased the damage of drought stress on plant. Spraying CaCl₂+ABAsignificantly promoted the positive effect of ABA, while EGTA+ABA and LaCl₃ +ABA didn’t show the promoting effect.

CHEN Lu-lu, WANG Xiu-feng, LIU Mei, YANG Feng-juan, SHI Qing-hua, WEI Min, LI Qing-ming. Effects of calcium and ABA on photosynthesis and related enzymes activities in cucumber seedlings under drought stress[J]. Chinese Journal of Applied Ecology, 2016, 27(12): 3996-4002.

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