Response of seed germination and seedling physiological characteristics of Medicago sativa to the simulated osmotic potential of PEG6000.

doi:10.13287/j.1001-9332.201709.025

Abstract

Abstract: The responses of seed germination and seedling physiological characteristics of alfalfa to different osmotic potentials were studied with the varied concentrations of PEG6000 (0%, 5%, 10%, 15%, 20%, 25% and 30% corresponding to osmotic potential of 0, -0.06, -0.17, -0.32, -0.53, -0.79 and -1.10 MPa, respectively) in this study. Five indexes of seed germination increased firstly and then decreased with a decrease of osmotic potential. Indexes of germination and vigor were the highest when osmotic potential was -0.06 MPa. Rate, potential and index germination reached the maximum when osmotic potential was -0.17 MPa. Seedling POD, SOD, CAT activities and chlorophyll content increased firstly and then decreased with a decrease of osmotic potential, and indexes reached the maximum when osmotic potential was -0.17 MPa. The ratio of chlorophyll a to chlorophyll b decreased firstly and then increased with a decrease of osmotic potential, and it reached the minimum when osmotic potential was -0.06 MPa. Pro, MDA and REC increased with a decrease of osmotic potential. Seed germination and the comprehensive indexes of seedling physiology were optimal for seed germination when osmotic potential was -0.06--0.17 MPa. Seed germination was restrained when the osmotic potential was lower than -0.79 MPa.

CHEN Shi-chao, WANG Meng, WANG Ji, GAO Yong, LIU Zong-qi, WANG Xiang. Response of seed germination and seedling physiological characteristics of Medicago sativa to the simulated osmotic potential of PEG6000.[J]. Chinese Journal of Applied Ecology, 2017, 28(9): 2923-2931.

References

[1] Li X-T (李兴桃). Research of Adaptability of three Species of Plants to Soil Water Potential. Master Thesis. Harbin: Northeast Forestry University, 2012 (in Chinese)
[2] Hegde DM. Effect of soil matric potential, method of irrigation and nitrogen fertilization on yield, quality, nutrient uptake and water use of radish (Raphanus sativus L.). Irrigation Science, 1987, 8: 13-22
[3] Zhang G-H (张光辉), Fei Y-H (费宇红), Wang H-J (王惠军), et al. Mechanism and forecasting threshold of saving water from irrigation in farmland on the basis of soil moisture potential variation. Earth Science Frontiers (地学前缘), 2010, 17(6): 174-180 (in Chinese)
[4] Wang D, Kang YH, Wan SQ. Effect of soil matric potential on tomato yield and water use under drip irrigation condition. Agricultural Water Management, 2007, 87: 180-186
[5] Shock CC, Wang FX. Soil water tension, a powerful measurement for productivity and stewardship. Hortscience, 2011, 46: 178-185
[6] Liu Y-C (刘玉春), Li J-S (李久生). Effects of lateral depths on alternative placements of tensiometers used for drip irrigation scheduling. Transactions of the Chinese Society of Agricultural Engineering (农业工程学报), 2010, 26(4): 18-24 (in Chinese)
[7] Yao N (姚宁), Zhou Y-G (周元刚), Song L-B (宋利兵), et al. Parameter estimation and verification of DSSAT-CERES-Wheat model forsimulation of growth and development of winter wheat under water stresses at different growth stages. Transactions of the Chinese Society of Agricultural Engineering (农业工程学报), 2015, 31(12): 138-150 (in Chinese)
[8] Bischoff A, Vonlanthen B, Steinger T, et al. Seed pro-venance matters: Effects on germination of four plant species used for ecological restoration. Basic and Applied Ecology, 2006, 7: 347-359
[9] Atree SM, Fowke LC. Embryogeny of gymnosperms: Advances in synthetic seed technology of conifers. Plant Cell, Tissue and Organ Culture, 1993, 35: 1-35
[10] Ma Z-L (马志良), Yang W-Q (杨万勤), Wu F-Z (吴福忠), et al. Effects of shading on the aboveground biomass and stiochiometry characteristics of Medicago sativa. Chinese Journal of Applied Ecology (应用生态学报), 2014, 25(11): 3139-3144 (in Chinese)
[11] Huo H-L (霍海丽), Wang Q (王琦), Zhang E-H (张恩和), et al. Effects of different water harvesting modes on alfalfa planting in semi-arid areas of Northwest China. Chinese Journal of Applied Ecology (应用生态学报), 2013, 24(10): 2770-2778 (in Chinese)
[12] Michel BE, Kaufmann MR. The osmotic potential of polyethylene glycol 6000. Plant Physiology, 1973, 51: 914-916
[13] Li H-S (李合生). Experimental Principle and Techno-logy in Plant Physiology and Biochemistry. Beijing: Higher Education Press, 2000 (in Chinese)
[14] Zhang Z-L (张志良). The Experiment Guidebook of Plant Physiology. Beijing: Higher Education Press, 1990 (in Chinese)
[15] Yang J-N (杨景宁), Wang Y-R (王彦荣). Effects of drought stress simulated by PEG on seed germination of four desert plant species. Acta Prataculturae Sinica (草业学报), 2012, 21(6): 23-29 (in Chinese)
[16] Liu J (刘佳), Xu C-X (徐昌旭), Cao W-D (曹卫东), et al. Study on drought resistance of 15 accessions of Astragalus sinicus L. germplasm materials at seed germination stage under PEG stress. Chinese Journal of Grassland (中国草地学报), 2012, 34(6): 18-25 (in Chinese)
[17] Liu G-H (刘贵河), Guo Y-P (郭郁频), Ren Y-X (任永霞), et al. Study on drought resistance of five kinds forage and crops during seed germination period under PEG stress. Seed (种子), 2013, 32(1): 15-19 (in Chinese)
[18] Heydecker W, Higgins J, Gulliver RL. Accelerated germination by osmotic seed treatment. Nature, 1973, 246: 42-44
[19] Liang G-L (梁国玲), Zhou Q-P (周青平), Yan H-B (颜红波), et al. Effect of polyethylene glycol (PEG) on seed germination characteristics of four species of Festuca. Pratacultural Science (草业科学), 2007, 24(6): 50-54 (in Chinese)
[20] Niu X-L (牛晓丽), Hu T-T (胡田田), Liu T-T (刘亭亭), et al. Appropriate partial water stress improving maize root absorbing capacity. Transactions of the Chinese Society of Agricultural Engineering (农业工程学报), 2014, 30(22): 80-86 (in Chinese)
[21] Gergely I, Korcak RF, Faust M. Polyethylene glycol induced water stress effects on apple seedlings. Ⅰ. Methodo-logy, water consumption, and dry matter production. Journal of the American Society for Horticultural Science, 1980, 105: 854-857
[22] Wu H-H (吴汉花), Cao X (曹雪), Jang F-L (蒋芳玲), et al. Effect of vigor index of non-heading Chinese cabbage seeds under PEG-6000 simulating drought, NaCl and temperature stress. Seed (种子), 2012, 31(7): 1-6 (in Chinese)
[23] Yu J (于军), Jiao P-P (焦培培). Inhibition of seed germination of Ammmopiptanthus nanus (M. Pop.) Cheng f. under simulated drought stress with polyethylene glycol (PEG6000). Genomics and Applied Biology (基因组学与应用生物学), 2010, 29(2): 355-360 (in Chinese)
[24] Wang H-N (王海宁), Zhang J-L (张建利), Feng L (冯林), et al. Effect of temperature and drought stress on seed germination of three forage species. Pra-tacultural Science (草业科学), 2009, 26(8): 87-92 (in Chinese)
[25] He F-L (何芳兰), Zhao M (赵明), Wang J-H (王继和), et al. Response to droughty stresses and drought resistances evaluation of seed germination of four desert vegetation. Arid Land Geography (干旱区地理), 2011, 34(1): 100-106 (in Chinese)
[26] Hua C (华春), Zhou Q-C (周泉澄), Zhang B-J (张边江), et al. Physiologic response of seedlings of Salicornia bigelovii Torr. and Salicornia europaea L. to drought simulated with PEG 6000. Arid Zone Research (干旱区研究), 2009, 26(5): 702-707 (in Chinese)
[27] Zhou H-B (周红兵), Wang Y-C (王迎春), Shi S-L (石松利), et al. Response of antioxidant system in leaves of Tetraena mongolica and Zygophyllum xantho-xylon seedlings to drought stress. Acta Botanica Boreali-Occidentalia Sinica (西北植物学报), 2011, 31(6): 1188-1194 (in Chinese)
[28] Yin Y-Q (尹永强), Hu J-B (胡建斌), Deng M-J (邓明军). Latest development of antioxidant system and responses to stress in plant leaves. Chinese Agricultural Science Bulletin (中国农学通报), 2007, 23(1): 105-110 (in Chinese)
[29] Chen Y-J (陈雅君), Zu Y-G (祖元刚), Liu H-M (刘慧民), et al. Effect of drought on membrane lipid peroxidation and protective enzymes activities in cells of different Kentucky bluegrass (Poa pratensis L.) cultivars. Chinese Journal of Grassland (中国草地学报), 2008, 30(5): 32-36 (in Chinese)
[30] Foyer CH, Lopez-Delgado H, Dat JF, et al. Hydrogen peroxide and glutathione-associated mechanisms of acclimatory stress tolerance and signalling. Physiologia Plantarum, 1997, 100: 241-254
[31] Feng H-F (冯慧芳), Xue L (薛立), Ren X-R (任向荣), et al. Physiological responses of four broadleaved seedlings to drought stress simulated by PEG. Acta Ecologica Sinica (生态学报), 2011, 31(2): 371-382 (in Chinese)
[32] Wan L-Q (万里强), Li X-L (李向林), Shi Y-H (石永红), et al. A study on the response and on the comparison of physiological and biochemical indexes of four Lolium perenne varieties under PEG stress. Acta Pratacu-lturae Sinica (草业学报), 2010, 19(1): 83-88 (in Chinese)
[33] Liang X-H (梁新华), Shi D-G (史大刚). Effect of drought stress on the content of malondialdehyole and activity of cell defense enzymes in Glycyrrhiza glabra seedlings roots. Agricultural Research in the Arid Areas (干旱地区农业研究), 2006, 24(3): 108-110 (in Chinese)
[34] Li M (李明), Wang G-X (王根轩). Effect of drought stress on activities of cell defense enzymes and lipid peroxidation in Glycyrrhiza uralensis seedlings. Acta Ecologica Sinica (生态学报), 2002, 22(4): 503-507 (in Chinese)
[35] Zhang S (张爽), Zhao C (赵纯), Dong R (董然), et al. Physiological responses of Mukdenia rossii under PEG-6000 drought stress. Northern Horticulture (北方园艺), 2013(22): 74-78 (in Chinese)
[36] Yang C-J (杨春杰), Zhang X-K (张学昆), Zou C-S (邹崇顺), et al. Effects of drought simulated by PEG-6000on germination and seedling growth of rapeseed (Brassica napus L.). Chinese Journal of Oil Crop Scien-ces (中国油料作物学报), 2007, 29(4): 425-430 (in Chinese)
[37] Zou C-J (邹春静), Han S-J (韩士杰), Xu W-D (徐文铎), et al. Eco-physiological responses of Picea mangolica ecotypes to drought stress. Chinese Journal of Applied Ecology (应用生态学报), 2003, 14(9): 1446-1450 (in Chinese)
[38] Yang N (杨宁), Wang C-L (王程亮), Li Y-S (李宜珅), et al. Physiological responses of Chorispora bungeanaseedings in vitro to drought stress simulated by PEG-6000. Guihaia (广西植物), 2015, 35(1): 77-83 (in Chinese)
[39] Hui Z-M (惠竹梅), Sun W-J (孙万金), Zhang Z-W (张振文). Effect of exogenous Ca²⁺on drought resis-tance physiological indexes of wine grape cultivar Pinot Noir under water stress. Journal of Northwest A&F University (Natural Science) (西北农林科技大学学报: 自然科学版), 2007, 35(9): 137-140 (in Chinese)
[40] Hom R, Paulsen H. Folding in vitro of light-harvesting chlorophyll a/b protein is coupled with pigment bin-ding. Journal of Molecular Biology, 2002, 318: 547-556
[41] Sun J-K (孙景宽), Zhang W-H (张文辉), Zhang J-M (张洁明), et al. Response to droughty stresses and drought resistances evaluation of four species during seed germination. Acta Botanica Boreali-Occidentalia Sinica (西北植物学报), 2006, 26(9): 1811-1818 (in Chinese)
[42] Xu Y-Z (许耀照), Zeng X-C (曾秀存), Wang Q-L (王勤礼), et al. Effects of PEG simulated drought stress on seed germination of different cucumber varieties. China Vegetables (中国蔬菜), 2010(14): 54-59 (in Chinese)