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Chinese Journal of Applied Ecology ›› 2017, Vol. 28 ›› Issue (9): 2961-2966.doi: 10.13287/j.1001-9332.201709.028

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Effects of Trichoderma hazianum and T. viride on seed germination and seedling growth of Chinese fir.

LI Yan-juan1,2, LIU Bo1,2, ZHUANG Zheng1,2, SHANG Tian-she1, LIU Qing-qing1,2, ZHU Chen-xi1,2, WANG Zheng-ning1,2*   

  1. 1College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2Engineering Research Center of Chinese Fir, State Forestry Administration, Fuzhou 350002, China.
  • Received:2017-02-22 Online:2017-09-18 Published:2017-09-18
  • Contact: * E-mail: wangzn@fafu.edu.cn
  • Supported by:

    This work was supported by the National Science Foundation of China (31570448, 31670714), and the New Century Excellent Talents Program of Fujian Province (K8015053A)

Abstract: Various concentrations of Trichoderma hazianum and T. viride treatments (0.003, 0.03, 0.3, 3, 30, 300 mg·L-1) were designed to investigate their effects on seed germination and seedling growth of Chinese fir. The results showed that seed germination and seedling growth were signifi-cantly affected by all concentrations of both Trichoderma, and they presented to increase and then fall with the increasing treatment concentrations. The seed germination rate, germination energy, germination index, vigor index, seedling root length, shoot height and fresh mass reached their maxima at the 0.03 mg·L-1 treatment. Compared with the control, the increments were 57.6%, 125.0%, 51.0%, 209.2%, 114.3%, 16.1% and 24.6% for T. hazianum, and 42.7%, 76.7%, 43.9%, 185.4%, 113.8%, 8.6% and 22.6% for T. viride, respectively. SOD and POD activities of Chinese fir seedlings reached their maxima, while MDA content reached the minimum at 0.3 mg·L-1 treatment. Compared with the control, SOD and POD increased by 157.6% and 179.9%, while MAD decreased by 86.1% for T. hazianum, and SOD and POD increased by 127.5% and 116.2%, while MAD decreased by 72.4% for T. viride. This study indicated that 0.03-0.3 mg·L-1 T. harzianum and T. viride could not only promote seed germination and seedling growth, but also improve the activities of antioxidant enzymes and the resistance of Chinese fir seedlings.