Abstract: To examine the effects of external glucose on growth, root architecture and nitrogen metabolism of Malus baccata seedlings in low carbon soil condition, Malus baccata seedlings were grown in sandy soil with the concentration of soil organic matter being 0.65%. The experiment consisted of three treatments: Control, with 2 g·kg^-1 glucose that equal to the ambient microbial biomass carbon (MBC), and with 10 g·kg^-1 glucose that was five times higher than the ambient MBC. The plant height, biomass, total root length and superficial area of the five times MBC group were 12.3%, 26.4%, 23.2% and 14.6% higher than that of the control, respectively. Root diameter, root volume and average diameter exhibited no significant difference under glucose treatments. The root activity was significantly increased under equal and five times MBC-glucose treatments, and reached its peak at 3 d and 15 d, about 119.1% and 75.7% higher than the control, respectively. Exogenous glucose addition significantly enhanced the concentrations of NO₃^-, NO₂^- and NH₄⁺ in roots. The activities of nitrate reductase, glutamine synthetase, glutamate dehydrogenase, glutamate synthase, glutamic-oxalacetic transaminase and glutamic-pyruvic transaminase were substantially increased in the roots, especially under five times MBC treatment. Five times higher than the ambient MBC of external carbon source promoted biomass accumulation, root growth, morphogenesis and N absorption of plants in low carbon sandy soil.