Abstract: This study was carried out to analyze the changes of growth, the contents of carbohydrates and soluble protein of Zoysia japonica clonal ramets under nutrient heterogeneity, where the connected and disconnected ramets were treated with different nutrient levels. The results indicated that under the nutrient heterogeneity the parent ramets in middle or high nutrient levels improved the aboveground biomass, belowground biomass, and total biomass, with the enhancement of 32.5%, 22.1% and 24.8% at high nutrient level, respectively, reduced the root/shoot ratio, the contents of soluble sugar and non-structural carbohydrates (NSC), with the reduction of 7.7%, 15.2% and 13.1% in high nutrient level, respectively, but had no significant impacts on the contents of starch, cellulous, and soluble protein of the connected daughter ramets. The daughter ramets in middle and high nutrient levels had no significant impacts on the growth and the contents of carbohydrates and soluble protein of the connected parent ramets. There was a significant physiological integration effects from parent to the daughter ramets on the biomass, root/shoot, the contents of soluble sugar and NSC. The intensity of physiological integration was proportional to the nutrient gradient of ramets, but had no significant physiological integration effects on the contents of starch, cellulous and soluble protein. The daughter ramets had no physiological integration for any indicator of the parent ramets. There was a unidirectional physiological integration between parent and daughter ramets of Z. japonica.