Combined remediation of polycyclic aromatic hydrocarbons (PAHs) by plant and immobilized bacteria in contaminated soil

doi:10.13287/j.1001-9332.202108.031

Abstract

Abstract: In this study, three dominant bacteria Cellulomonas flavigena (Ⅰ), Cellulomonas flavigena (Ⅱ), Sphingomonas paucimobilis (Ⅲ) from Fire Phoenix rhizosphere soil were used to develop a multi-microbial agent system. For oil-contaminated soil in the Dagang oilfield, the immobilized test bacteria were inoculated into the Fire Phoenix rhizosphere soil to examine the effects of bacterial agents on polycyclic aromatic hydrocarbons (PAHs)-contaminated soil. The results showed that PAHs degradation was promoted under the ⅠⅢ (with an effective number of viable bacteria of 10⁹ cfu·mL^-1) and ⅠⅡⅢ (with an effective number of viable bacteria of 10⁷ cfu·mL^-1) treatments. The PAHs degradation rates were 32.2% and 41.4%, respectively, being significantly higher than that in the control treatments. The ⅠⅡⅢ treatment significantly promoted the belowground biomass of Fire Phoenix, which was 31.2% higher than that of the control treatment. Our results suggested that the multi-microbial agent constructed by the three dominant bacteria ⅠⅡⅢ could be used as a strengthening method for the remediation of PAHs-contaminated soil by Fire Phoenix, which provided a novel method for microbial enhanced phytoremediation technology.

Key words: immobilized multi-microbial agent, Fire Phoenix, plant-microorganism combined remediation, degradation rate of polycyclic aromatic hydrocarbon (PAHs), oil-contaminated soil

LI Na, LIU Rui, TAI Pei-dong, ZHOU Xing-xing, XIAO Yao, LI Ling-mei, HOU Li-qun, ZHENG Ting-yu. Combined remediation of polycyclic aromatic hydrocarbons (PAHs) by plant and immobilized bacteria in contaminated soil[J]. Chinese Journal of Applied Ecology, 2021, 32(8): 2982-2988.

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