Community characteristics and functions of phosphate-solubilizing bacteria in rhizosphere soil of natural and planted Pinus tabuliformis forests on the Loess Plateau, Northwest China

doi:10.13287/j.1001-9332.202109.006

Abstract

Abstract: To investigate the interaction between Pinus tabuliformis and root microorganisms and its effects on the stability of P. tabuliformis forests, we collected rhizosphere and non-rhizosphere soil from natural and planted P. tabuliformis forests in Huanglong County of Shaanxi Province. The non-rhizosphere soil was used to analyze chemical properties, while the rhizosphere soil was used to isolate and purify phosphorus-solubilizing (organic and inorganic phosphorus) bacteria. We mea-sured the phosphate-solubilizing ability and identified those bacteria using DNA genes sequencing. The results showed that soil total carbon concentration (TC), total nitrogen concentration (TN), carbon:nitrogen (C/N), and nitrogen:phosphorus (N/P) were significantly higher in natural forest than those in the plantation. A total of 20 genera and 65 species of phosphate-solubilizing bacteria were identified in those two forests, with Bacillus, Streptomyces and Pseudomonas as the dominant group. The diversity, richness, and evenness of phosphate-solubilizing bacteria in the natural forest were higher than that in the planted forest, but dominance was lower. Streptomyces was positively correlated with soil TC, TN, C/N and N/P ratios, while Bacillus and Pseudomonas were positively correlated with soil nitrate (NO₃^-), ammonium (NH₄⁺), available phosphorus (AP), and total phosphorus (TP). The solubilizing ability of phosphate-solubilizing bacteria was different between two forest types, indicating that Pseudomonas sp.34-5 shared in two forests had the strongest phosphate-solubilizing ability for calcium phosphate with 11.40 μg·mL^-1 and Bacillus mycoides BF1-5 exclusive to natural forest had the strongest phosphate-solubilizing ability for lecithin with 4.58 μg·mL^-1. The composition and structure of phosphorus-solubilizing bacterial community were different in two forest types, with higher diversity, richness and phosphate-solubilizing ability in natural forest than that in plantation.

Key words: Loess Plateau, Pinus tabuliformis, phosphate-solubilizing bacteria, phosphate-solubilizing ability

ZHU Ying, KU Yong-li, LIU Jin-liang, Le Thi Hien, ZHAO Zhong. Community characteristics and functions of phosphate-solubilizing bacteria in rhizosphere soil of natural and planted Pinus tabuliformis forests on the Loess Plateau, Northwest China[J]. Chinese Journal of Applied Ecology, 2021, 32(9): 3097-3106.

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