C/N ratio-induced changes in predominant bacteria affected carbon/nitrogen losses and humus synthesis in composting

doi:10.13287/j.1001-9332.202111.041

Abstract

Abstract: To explore how C/N ratio-induced changes in the predominant microbial succession in the composting microbiota affect C and N losses, and promote the formation of humic substance, a composting experiment was conducted with C/N ratio of 20:1, 25:1 and 30:1. Results showed that N and C losses under 30:1 and 25:1 treatments were 33.5%, 18.9% and 23.6%, 10.8% lower than those of the treatment of 20:1, respectively. Redundancy analysis results suggested that higher C/N ratio promoted nitrogen fixing bacteria, while inhibited the proliferation of denitrifying bacte-ria, resulting in reduction of the losses of C and N during composting. Higher C/N ratio significantly promoted the growth and reproduction of lignocellulose degrading bacteria for the degradation of fulvic acid and humin to form more humic acid, and improved the polymerization of humic substance. Therefore, C/N ratio could significantly affect key microbes during composting, with consequences on the process and quality. It could be an effective way to regulate C and N losses, promote HS formation, and eventually improve the compost quality and reduce the secondary environmental pollution during composting.

Key words: composting, C and N losses, humic substances, nitrogen fixing bacteria, denitrifying bacteria, lignocelluloses-degrading microbes.

ZHANG Wen-ming, WANG Xu-jie, YIN Si-qian, CHANG Xin-yi, HAI Long. C/N ratio-induced changes in predominant bacteria affected carbon/nitrogen losses and humus synthesis in composting[J]. Chinese Journal of Applied Ecology, 2021, 32(11): 4119-4128.

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