Identification of released population of Japanese flounder based on stable isotopes analysis

doi:10.13287/j.1001-9332.202201.023

Abstract

Abstract: Japanese flounder (Paralichthys olivaceus) is an important releasing fish in the Yellow and Bohai Seas of China. The identification of wild and releasing population is the premise to evaluate the enhancement effects. In order to study the application of stable isotope in the identification of released P. olivaceus population, captured juveniles in the offshore releasing area of Qinhuangdao were distinguished into wild and released population using previous method (combination of morphology and molecular). Then, the contents of carbon and nitrogen stable isotope in muscle and otoliths (including the whole and the core region) were measured. The cultured population was set as control. The results showed that δ¹³C values (wild population: -17.19‰±0.73‰; released population: -17.10‰±0.61‰; cultured population: -20.75‰±0.07‰) and δ¹⁵N values (wild population: 11.81‰±0.38‰; released population: 11.62‰±0.48‰; cultured population: 8.64‰±0.60‰) of muscle and δ¹³C value (wild popu-lation: -4.47‰±0.35‰; released population: -4.63‰±0.29‰; cultured population: -6.59‰±0.58‰) of the whole otolith could be used to identify the cultured population, but could not be used to distinguish the wild from the released population. The δ¹³C value (wild population: -4.66‰±0.30‰; released population: -5.41‰±0.21‰; cultured population: -5.37‰±0.19‰) of the core region of otolith could be used to identify the wild popu-lation. The δ¹⁸O values of the whole and the core region of otolith from these three groups were overlapped and could not be used to distinguish different populations. Our results indicated that the δ¹³C value of the core area of otolith could be used to identify wild and released population, with application prospect in the identification of broodstocks participating in spawning migration. This study provided basic data and technical methods for evaluating early resources replenishment and the effects of Japanese flounder enhancement.

Key words: stable isotope, Japanese flounder, wild population, released population, cultured population, otolith, identification

WANG Qing-lin, YU Shan-shan, JIN Xiao-min, REN Jian-gong, SI Fei, SUN Gui-qing, SUN Zhao-hui, BAI Zeng-qi. Identification of released population of Japanese flounder based on stable isotopes analysis[J]. Chinese Journal of Applied Ecology, 2022, 33(1): 261-267.

References 31

[1]	Newman SJ, Pember MB, Rome BM, et al. Stock structure of blue threadfin Eleutheronema tetradactylum across northern Australia as inferred from stable isotopes in sagittal otolith carbonate. Fisheries Management and Ecology, 2011, 18: 246-257
[2]	Høie H, Folkvord A. Estimating the timing of growth rings in Atlantic cod otoliths using stable oxygen isotopes. Journal of Fish Biology, 2006, 68: 826-837
[3]	Geffen AJ. Otolith oxygen and carbon stable isotopes in wild and laboratory-reared plaice (Pleuronectes platessa). Environmental Biology of Fishes, 2012, 95: 419-430
[4]	Ciner B, Wang Y, Parker W. Oxygen isotopic variations in modern cetacean teeth and bones: Implications for ecological, paleoecological, and paleoclimatic studies. Science Bulletin, 2016, 61: 92-104
[5]	谭鲁玉, 王玉堃, 唐学玺, 等. 黄海小黄鱼不同组织中δ15N的分布特征及其生态学意义. 渔业科学进展, 2018, 39(3): 30-35
[6]	Rounick JS, Winterbourn MJ. Stable carbon isotopes and carbon flow in ecosystems. Bioscience, 1986, 36: 171-177
[7]	Campana SE, Neilson JD. Microstructure of fish otoliths. Canadian Journal of Fisheries and Aquatic Sciences, 1985, 42: 1014-1032
[8]	徐永江, 柳学周, 张凯, 等. 编码金属标签对牙鲆(Paralichthys olivaceus)苗种标记的效果. 渔业科学进展, 2017, 38(1): 168-174
[9]	刘奇. 褐牙鲆标记技志与增殖放流试验研究. 硕士论文. 青岛: 中国海洋大学, 2009
[10]	于广宝, 李刚. 牙鲆生态标记放流鱼苗培育技术. 中国水产, 2016(7): 102-105
[11]	朱学武, 徐永江, 柳学周, 等. 池塘养殖牙鲆(Paralichthys olivaceus)无眼侧体色黑化消褪机理. 渔业科学进展, 2017, 38(1): 103-110
[12]	童爱萍, 司飞, 刘海金, 等. mtDNA和微卫星标记在放流牙鲆和非放流牙鲆鉴定中的应用. 中国水产科学, 2015, 22(4): 630-637
[13]	Wang QL, Si F, Yu SS, et al. Identification and adaptability of hatchery-origin Japanese flounder released into the Bohai Sea after the summer fishing moratorium. Aquaculture, 2019, 504: 164-171
[14]	罗刚, 庄平, 赵峰, 等. 我国水生生物增殖放流物种选择发展现状、存在问题及对策. 海洋渔业, 2016, 38(5): 551-560
[15]	司飞, 任建功, 王青林, 等. 基于浸泡法的牙鲆耳石锶标记技术研究. 中国水产科学, 2019, 26(3): 534-545
[16]	司飞, 王青林, 于清海, 等. 基于投喂法的牙鲆耳石锶标记. 渔业科学进展, 2019, 40(4): 65-72
[17]	Haga Y, Takeuchi T, Murayama Y, et al. Vitamin D3 compounds induce hypermelanosis on the blind side and vertebral deformity in juvenile Japanese flounder Paralichthys olivaceus. Fishery Science, 2004, 70: 59-67
[18]	Tomiyama T, Mizuno T, Watanabe M, et al. Patterns and frequency of hypermelanosis on the blind side in wild Japanese flounder. Nippon Suisan Gakkaishi, 2008, 74: 171-176
[19]	姜涛, 刘洪波, 杨健. 长江口刀鲚幼鱼耳石碳、氧同位素特征初报. 海洋科学, 2015, 39(6): 48-53
[20]	Yokoyama H, Tamaki A, Harada K, et al. Variability of diet-tissue isotopic fractionation in estuarine macro-benthos. Marine Ecology Progress Series, 2005, 296: 115-128
[21]	DeNiro MJ, Epstein S. Influence of diet on the distribution of carbon isotopes in animals. Geochimica et Cosmochimica Acta, 1978, 42: 495-506
[22]	Luo RJ, Jiang T, Chen XB, et al. Determination of geographic origin of Chinese mitten crab (Eriocheir sinensis) using integrated stable isotope and multi-element analyses. Food Chemistry, 2019, 274: 1-7
[23]	Zhang XF, Liu Y, Li Y, et al. Identification of the geographical origins of sea cucumber (Apostichopus japonicus) in northern China by using stable isotope ratios and fatty acid profiles. Food Chemistry, 2017, 218: 269-276
[24]	Berejikian BA, Hard JJ, Tatara CP, et al. Rearing strategies alter patterns of size-selective mortality and heritable size variation in steelhead trout (Oncorhynchus mykiss). Canadian Journal of Fisheries and Aquatic Sciences, 2017, 74: 273-283
[25]	朱国平. 金枪鱼类耳石微化学研究进展. 应用生态学报, 2011, 22(8): 2211-2218
[26]	Gibson SM, Patterson WF, Phelps RP, et al. Distinguishing wild from hatchery-produced juvenile red snapper with otolith chemical signatures. North American Journal of Fisheries Management, 2010, 30: 1176-1186
[27]	Tomida Y, Suzuki T, Yamada T, et al. Differences in oxygen and carbon stable isotope ratios between hatchery and wild pink salmon fry. Fisheries Science, 2014, 80: 273-280
[28]	Tanner SE, Reis-Santos P, Cabral HN. Otolith chemistry in stock delineation: A brief overview, current challenges and future prospects. Fisheries Research, 2016, 173: 206-213
[29]	Schwarcz HP, Gao YW, Campana SE, et al. Stable carbon isotope variations in otoliths of Atlantic cod (Gadus morhua). Canadian Journal of Fisheries and Aquatic Sciences, 1998, 55: 1798-1806
[30]	高永文, 鲁安怀, 王清印, 等. 鱼群识别的氧、碳稳定同位素方法. 矿物岩石地球化学通报, 2011, 30(3): 270-276
[31]	何勇凤, 吴兴兵, 王旭歌, 等. 四川裂腹鱼耳石碳、氧稳定同位素特征. 应用生态学报, 2017, 28(7): 2339-2343