Effects of different carbon addition modes on the soil priming effect of a subtropical Phyllostachys edulis forest under nitrogen deposition

doi:10.13287/j.1001-9332.202210.015

Abstract

Abstract: Priming effect (PE) plays an important role in regulating terrestrial soil carbon (C) cycling, but the impact of different C addition modes on the PE in subtropical forest ecosystems with increasing nitrogen (N) deposition is unclear. In this study, we investigated the effects of C addition patterns (single or repeated C addition) on soil PE by adding ¹³C-labeled glucose for 90 d in an incubation experiment with different levels of N application (0, 20, and 80 kg N·hm^-2·a^-1). The different patterns of glucose addition significantly increased soil organic C (SOC) mineralization and produced positive PE. Single glucose addition resulted in stronger PE than repeated addition. PE was significantly weakened with increasing N application levels, indicating that N deposition inhibited soil excitation in Phyllostachys edulis forests. The cumulative PE was significantly negatively correlated with β-N-acetylaminoglucosidase (NAG) and peroxidase (PEO) activities, and was significantly positively correlated with microbial biomass P (MBP) and potential of hydrogen (pH). Our findings indicated that, when acting together on soil, N application and C addition could strongly affect soil C stocks by stimulating the mineralization of native soil organic matter in subtropical forests. The findings further indicated that single C addition model might overestimate the effect of exogenous readily decomposable organic C on PE and ignore the effect of N deposition on PE, which in turn would overestimate the mineralization loss of forest SOC.

Key words: priming effect, nitrogen deposition, carbon addition mode, enzymatic activity

XU Min, LIU Yuan-yuan, YUAN Xiao-cun, ZENG Quan-xin, LIN Hui-ying, WU Xiao-xia, CUI Ju-yan, CHEN Wen-wei, CHEN Yueh-min. Effects of different carbon addition modes on the soil priming effect of a subtropical Phyllostachys edulis forest under nitrogen deposition[J]. Chinese Journal of Applied Ecology, 2022, 33(10): 2619-2627.

References

[1] Tarnocai C, Canadell JG, Schuur EAG, et al. Soil organic carbon pools in the northern circumpolar permafrost region. Global Biogeochemical Cycles, 2009, 23: 2607-2617
[2] Blagodatskaya EV, Kuzyakov Y. Mechanisms of real and apparent priming effects and their dependence on soil microbial biomass and community structure: Critical review. Biology and Fertility of Soils, 2008, 45: 115-131
[3] Kuzyakov Y, Friedel JK, Stahr K. Review of mechanisms and quantiﬁcation of priming effects. Soil Biology and Biochemistry, 2000, 32: 1485-1498
[4] Zhang H, Ding W, Luo J, et al. Temporal responses of microorganisms and native organic carbon mineralization to ¹³C-glucose addition in a sandy loam soil with long-term fertilization. European Journal of Soil Biology, 2016, 74: 16-22
[5] Mehnaz KR, Corneo PE, Keitel C, et al. Carbon and phosphorus addition effects on microbial carbon use efficiency, soil organic matter priming, gross nitrogen mineralization and nitrous oxide emission from soil. Soil Biology and Biochemistry, 2019, 134: 175-186
[6] Ramirez KS, Lauber CL, Knight R, et al. Consistent effects of nitrogen fertilization on soil bacterial communities in contrasting systems. Ecology, 2010, 91: 3463-3470
[7] Galloway JN, Townsend AR, Erisman JW, et al. Transformation of the nitrogen cycle: Recent trends, questions, and potential solutions. Science, 2008, 320: 889-892
[8] Vet R, Artz RS, Carou S, et al. A global assessment of precipitation chemistry and deposition of sulfur, nitrogen, sea salt, base cations, organic acids, acidity and pH, and phosphorus. Atmospheric Environment, 2014, 93: 3-100
[9] Meyer N, Welp G, Rodionov A, et al. Nitrogen and phosphorus supply controls soil organic carbon mineralization in tropical topsoil and subsoil. Soil Biology and Biochemistry, 2018, 119: 152-161
[10] Zhou FW, Cui J, Zhou J, et al. Increasing atmospheric deposition nitrogen and ammonium reduced microbial activity and changed the bacterial community composition of red paddy soil. Science of the Total Environment, 2018, 633: 776-784
[11] Chen R, Senbayram M, Blagodatsky S, et al. Soil C and N availability determine the priming effect: Microbial N mining and stoichiometric decomposition theories. Global Change Biology, 2014, 20: 2356-2367
[12] Qiao NA, Schaefer D, Blagodatskaya E, et al. Labile carbon retention compensates for CO₂ released by pri-ming in forest soils. Global Change Biology, 2014, 20: 1943-1954
[13] Feng J, Tang M, Zhu B. Soil priming effect and its responses to nutrient addition along a tropical forest elevation gradient. Global Change Biology, 2021, 27: 2793-2806
[14] Bastida F, García C, Fierer N, et al. Global ecological predictors of the soil priming effect. Nature Communications, 2019, 10: 3481
[15] Hamer U, Marschner B. Priming effects in soils after combined and repeated substrate additions. Geoderma, 2005, 128: 38-51
[16] Hicks LC, Meir P, Nottingham AT, et al. Carbon and nitrogen inputs differentially affect priming of soil orga-nic matter in tropical lowland and montane soils. Soil Biology and Biochemistry, 2019, 129: 212-222
[17] Kuzyakov Y, Hill PW, Jones DL. Root exudate components change litter decomposition in a simulated rhizosphere depending on temperature. Plant and Soil, 2007, 290: 293-305
[18] Wang Q, Chen L, Yang Q, et al. Different effects of single versus repeated additions of glucose on the soil organic carbon turnover in a temperate forest receiving long-term N addition. Geoderma, 2019, 341: 59-67
[19] Song XZ, Zhou GM, Jiang H, et al. Carbon sequestration by Chinese bamboo forests and their ecological benefits: Assessment of potential, problems, and future challenges. Environmental Reviews, 2011, 19: 418-428
[20] 袁磊, 李文周, 陈文伟, 等. 戴云山国家级自然保护区大气氮沉降特点. 环境科学, 2016, 37(11): 4142-4146
[21] Jia Y, Yu G, Gao Y, et al. Global inorganic nitrogen dry deposition inferred from ground- and space-based measurements. Scientific Reports, 2016, 6: 19810
[22] 程蕾, 林开淼, 周嘉聪, 等. 氮沉降对毛竹林土壤可溶性有机质数量与光谱学特征的影响. 应用生态学报, 2019, 30(5): 1754-1762
[23] Carter MR. Soil Sampling and Methods of Analysis. Boca Raton, FL, USA: The Chemical Rubber Company Press, 1993
[24] 鲍士旦. 土壤农化分析. 第三版. 北京: 中国农业出版社, 2000
[25] Vance ED, Brookes PC, Jenkinson DS. An extraction method for measuring soil microbial biomass C. Soil Biology and Biochemistry, 1987, 19: 703-707
[26] Saiya-Cork KR, Sinsabaugh RL, Zak DR. The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biology and Biochemistry, 2002, 34: 1309-1315
[27] Van Zwieten L, Kimber S, Morris S, et al. Influence of biochars on flux of N₂O and CO₂ from Ferrosol. Austra-lian Journal of Soil Research, 2010, 48: 555-568
[28] Aylward GH, Finlay TJV, eds. SI Chemical Data. 2nd. New York: John Wiley and Sons, 1973
[29] Li XJ, Xie JS, Zhang QF, et al. Substrate availability and soil microbes drive temperature sensitivity of soil organic carbon mineralization to warming along an elevation gradient in subtropical Asia. Geoderma, 2020, 364: 114198
[30] Keith A, Singh B, Singh BP. Interactive priming of biochar and labile organic matter mineralization in a smectite-rich soil. Environmental Science and Technology, 2011, 45: 9611-9618
[31] Fontaine S, Henault C, Aamor A, et al. Fungi mediate long term sequestration of carbon and nitrogen in soil through their priming effect. Soil Biology and Biochemistry, 2011, 43: 86-96
[32] Craine JM, Morrow C, Fierer N. Microbial nitrogen limi-tation increases decomposition. Ecology, 2007, 88: 2105-2113
[33] Garcia-Pausas J, Paterson E. Microbial community abundance and structure are determinants of soil organic matter mineralisation in the presence of labile carbon. Soil Biology and Biochemistry, 2011, 43: 1705-1713
[34] Dijkstra FA, Carrillo Y, Pendall E, et al. Rhizosphere priming: A nutrient perspective. Frontiers in Microbiology, 2013, 4: 216
[35] Eberwein JR, Oikawa PY, Allsman LA, et al. Carbon availability regulates soil respiration response to nitrogen and temperature. Soil Biology and Biochemistry, 2015, 88: 158-164
[36] Keuskamp JA, Feller IC, Laanbroek HJ, et al. Short- and long-term effects of nutrient enrichment on microbial exoenzyme activity in mangrove peat. Soil Biology and Biochemistry, 2015, 81: 38-47
[37] Fang XM, Zhang XL, Chen FS, et al. Phosphorus addition alters the response of soil organic carbon decomposition to nitrogen deposition in a subtropical forest. Soil Biology and Biochemistry, 2019, 133: 119-128
[38] Sinsabaugh RL. Phenol oxidase, peroxidase and organic matter dynamics of soil. Soil Biology and Biochemistry, 2010, 42: 391-404
[39] Wang Q, Wang Y, Wang S, et al. Fresh carbon and nitrogen inputs alter organic carbon mineralization and microbial community in forest deep soil layers. Soil Bio-logy and Biochemistry, 2014, 72: 145-151
[40] Chen L, Liu LI, Qin S, et al. Regulation of priming effect by soil organic matter stability over a broad geographic scale. Nature Communications, 2019, 10: 1-10
[41] Fang Y, Nazaries L, Singh BK, et al. Microbial mechanisms of carbon priming effects revealed during the interaction of crop residue and nutrient inputs in contrasting soils. Global Change Biology, 2018, 24: 2775-2790
[42] Sun Z, Liu S, Zhang T, et al. Priming of soil organic carbon decomposition induced by exogenous organic carbon input: A meta-analysis. Plant and Soil, 2019, 443: 463-471
[43] 李九玉, 邓开英, 章威, 等. 添加葡萄糖对红壤农田肥料氮转化及其酸化的影响. 土壤学报, 2021, 58(1): 162-168